In order to clarify the immunological and genetic features of functional
Mhc class IIβ genes in chicken and quails, we established a modified RT-PCR method, which classify to major or minor
B-Lβ genes. Using this methodlogy, we classified quail
Mhc class IIβ (
CojaIIβ ) sequences to major or minor
CojaIIβ, and found interesting features of functional
MhcIIβ genes by detail comparisons between the quail and chicken, and the quail H
TUA and L
TUA lines. First, one major and one minor
B-Lβ genes was identified in the chicken B
21 haplotype. Four major and six minor
CojaIIβ genes in H
TUA line and two major and seven minor
CojaIIβ genes in L
TUA line were identified in quail two lines, respectively. Major genes were highly expressed in most of organs but minor genes were lowly expressed in a part of organs. Meanwhile, minor
CojaIIβ genes weakly expressed only in some immune organs, and this phenomenon suggest that these genes specifically appears depending on the modality and maturity of lymphocytes.
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