Latest Volume/Issue

Volume And Issue List
Drug Delivery System
- Vol.31(2016)
  - No.2 p.107-
  - No.1 p.3-
- Vol.30(2015)
- Vol.29(2014)
- Vol.28(2013)
- Vol.27(2012)
- Vol.26(2011)
- Vol.25(2010)
- Vol.24(2009)
- Vol.23(2008)
- Vol.22(2007)
- Vol.21(2006)
- Vol.20(2005)
- Vol.19(2004)
- Vol.18(2003)
- Vol.17(2002)
- Vol.16(2001)
- Vol.15(2000)
- Vol.14(1999)
- Vol.13(1998)
- Vol.12(1997)
- Vol.11(1996)
- Vol.10(1995)
- Vol.9(1994)
- Vol.8(1993)
- Vol.7(1992)
- Vol.6(1991)
- Vol.5(1990)
- Vol.4(1989)
- Vol.3(1988)
- Vol.2(1987)
  - No.2 p.100-
  - No.1 p.2-
- Vol.1(1986)
  - No.1 p.2-

Drug Delivery System Vol. 31(2016) No. 2
Functional analysis in cells and animals based on live imaging

[Feature articles] “Functional analysis in cells and animals based on live imaging” Editor:Yuriko Higuchi

[FOREWORD] Development of drug delivery system and imaging technique

Yuriko Higuchi

Released: June 25, 2016

p.107

Full Text PDF[153K]

[OPINION] There's plenty of room for fluorescence microscopy

Takashi Funatsu

Released: June 25, 2016

p.108

Full Text PDF[165K]

Analysis of cellular functions by specific fluorescence labeling and imaging of mRNAs in living cells

Takashi Funatsu

Released: June 25, 2016

p.110-118

Imaging of mRNAs in living cells is an effective approach to elucidate the molecular mechanism of biomolecules and cellular functions. Here we introduce methods to fluorescently label a specific mRNA using antisense oligonucleotide probes or RNA binding proteins. Next, we introduce examples of imaging dynamics of mRNAs in living cells. The first example is the analysis of dynamics of mRNAs in stress granules(SGs). mRNAs in COS7 cells were labeled with a poly(U)₂₂ 2'-O-methyl RNA and SGs were formed by arsenite. The analysis of mRNA dynamics using FRAP showed that approximately one-third of the endogenous mRNAs in SGs was immobile, another one-third was diffusive, and the remaining one-third was in equilibrium between binding to and dissociating from SGs, with a time constant of approximately 300 seconds. Our results revealed the behavior of endogenous mRNAs, and indicated that SGs act as dynamic harbors of untranslated poly(A)⁺ mRNAs. The second example is single-molecule imaging of β-actin mRNAs in a chicken fibroblast. β-Actin mRNAs were labeled with MS2-GFP and their movement was analyzed. Singe-molecule tracking of individual mRNAs revealed that the majority of mRNAs were in unrestricted Brownian motion at the leading edge and in restricted Brownian motion in the perinuclear region. The macroscopic diffusion coefficient of mRNA(D_MACRO) at the leading edge was 0.3μm²/s. On the other hand, D_MACRO in the perinuclear region was 0.02μm²/s. The destruction of microfilaments with cytochalasin D led to an increase in D_MACRO to 0.2μm²/s in the perinuclear region. These results suggest that the microstructure, composed of microfilaments, serves as a barrier for the movement of β-actin mRNA.

The current trends and future prospect of neural activity measurement by genetically-encoded voltage indicators

Shigenori Inagaki, Takeharu Nagai

Released: June 25, 2016

p.119-126

Genetically-Encoded Voltage Indicators (GEVIs) draw neuroscientist's attention since it enables to simultaneously monitor direct electrical activity from lots of neurons, and which is not achievable by using electrodes. However many GEVIs reported so far often confuse users. Actually since each GEVIs has pros and cons, we have to carefully select GEVIs which is the most suitable for one's experiments according to the accurate information. Here, we describe the trend of GEVIs from ion channel based GEVIs reported as prototypes in the early stage to current rhodopsin-fluorescent protein based GEVIs. Also we note the feature of representative GEVIs and which GEVIs should be used for one's experiments.

Photo-controllable nitric oxide releasers and biological applications

Hidehiko Nakagawa

Released: June 25, 2016

p.127-134

Nitric oxide(NO) is now recognized as an endogenous signaling molecule involved in various physiological events such as vasodilation. Since NO is a gaseous molecule and has a short half-life due to high chemical reactivity under ambient conditions, NO itself is practically hard to be handled in biological systems. So, NO chemical donors which release NO under physiological conditions are now indispensable chemical tools in NO biological research. Especially, “caged” NO, which releases NO in response to photoirradiation, are advantageous in NO treatment with spatiotemporal control. We have been developed caged NOs, which have unique photochemical reaction mechanisms for NO release, and adopted them to tissue and in vivo experiments. It was demonstrated that some are responsible for visible light and applicable cellular and tissue experiments, and others are responsible to near infrared pulse laser and applicable for in vivo experiments.

The role of in vivo fluorescence imaging of transplanted stem cells in regenerative medicine drug discovery research

Hiroshi Yukawa, Yoshinobu Baba

Released: June 25, 2016

p.135-145

Full Text PDF[1677K]

Intravital imaging of live mice at cellular resolution

Sota Yamada, Yuriko Higuchi, Mitsuru Hashida

Released: June 25, 2016

p.146-153

Cellular or molecular dynamics and function are continuously changing in vivo. Their properties also vary under the influence of tissue micro-environment. Dynamic information of a cell or a molecule obtained through intravital imaging allows spatio-temporal analysis of various phenomena in a living body. However, a common hurdle in achieving high resolution imaging is the motion artifact which is caused by motion of organs, such as breathing, cardiac contractions, pulsatile blood flow or peristalsis. In this section, we will introduce the techniques for the fluorescence imaging in a living mouse in real time at cellular level resolution.

[Serial] Front line of DDS development in pharmaceutical industries

Exenatide extended-release for injectable suspension, BYDUREON^® 2mg Pen

Akihiro Kusaba

Released: June 25, 2016

p.156-161

Exenatide extended-release for injectable suspension, BYDUREON^® 2mg, an injectable glucagon-like peptide-1(GLP-1)receptor agonist, has been shown to improve glycemic control with positive effect on body weight in patients with type 2 diabetes. This long acting formulation contains exenatide encapsulated in microspheres of PLG. It is injected subcutaneously by patients once weekly, with no dose titration required. However, it was complicated to prepare for injection. Therefore, BYDUREON^® 2mg Pen, a single-use, dual-chamber pen was developed to improve the convenience of exenatide once weekly delivery.

[Serial] Reviews on useful reagents for DDS research and development

MRI contrast agent（gadolinium based MRI contrast agent）

Kouichi Shiraishi

Released: June 25, 2016

p.164-166

Full Text PDF[366K]

“Young square”(mini review)

Promotion of Nano-safety science toward ensuring the safety of nanomedicine

Kazuma Higashisaka

Released: June 25, 2016

p.168-169

Full Text PDF[366K]

1 - 10 / 10 Articles.

Top of this Page

System Maintenance

J-STAGE will undergo scheduled maintenance as follows.
Year 2016
Oct 29(Sat) 10:00 - 15:00(JST)
(Oct 29(Sat) 01:00 - 06:00(UTC))

Details

Announcement From J-STAGE

May 18, 2016
We have released “J-STAGE BETA site”.

May 01, 2015
Please note the "spoofing mail" that pretends to be J-STAGE.

Back Issues

Volume And Issue List
Drug Delivery System

Drug Delivery System Vol. 31(2016) No. 2
Functional analysis in cells and animals based on live imaging

[Feature articles] “Functional analysis in cells and animals based on live imaging” Editor:Yuriko Higuchi

[FOREWORD] Development of drug delivery system and imaging technique

[OPINION] There's plenty of room for fluorescence microscopy

Analysis of cellular functions by specific fluorescence labeling and imaging of mRNAs in living cells

The current trends and future prospect of neural activity measurement by genetically-encoded voltage indicators

Photo-controllable nitric oxide releasers and biological applications

The role of in vivo fluorescence imaging of transplanted stem cells in regenerative medicine drug discovery research

Intravital imaging of live mice at cellular resolution

[Serial] Front line of DDS development in pharmaceutical industries

Exenatide extended-release for injectable suspension, BYDUREON^® 2mg Pen

[Serial] Reviews on useful reagents for DDS research and development

MRI contrast agent（gadolinium based MRI contrast agent）

“Young square”(mini review)

Promotion of Nano-safety science toward ensuring the safety of nanomedicine

System Maintenance

Announcement From J-STAGE

Journal Tools

Share this Title

Volume And Issue List Drug Delivery System

Drug Delivery System Vol. 31(2016) No. 2 Functional analysis in cells and animals based on live imaging

[Feature articles] “Functional analysis in cells and animals based on live imaging” Editor:Yuriko Higuchi

[Serial] Front line of DDS development in pharmaceutical industries

[Serial] Reviews on useful reagents for DDS research and development

“Young square”(mini review)

System Maintenance

Announcement From J-STAGE

Journal Tools

Share this Title

Volume And Issue List
Drug Delivery System

Drug Delivery System Vol. 31(2016) No. 2
Functional analysis in cells and animals based on live imaging