This study was conducted to evaluate the role of salicylic acid (SA) on acute ozone (O
3: 0, 0.1, and 0.3 cm
3 m
−3; O
0, O
0.1 and O
0.3, respectively)-induced photosynthetic inhibition of paddy rice leaves given different atmospheric carbon dioxide concentrations (CO
2: 400 and 800 cm
3 m
−3; C
400 and C
800, respectively). Salicylic acid solutions (0, 0.1, and 1 mM; S
0, S
0.1, and S
1, respectively) were applied as a pretreatment one day before O
3 exposure. Gas exchange, chlorophyll fluorescence, and ascorbic acid were measured immediately before (BE), immediately after (AE-0), and 1 d and 3 d after (AE-1, AE-3) 5-h O
3 exposure. The photosynthesis-related parameters, total ascorbic acid, and redox state of ascorbic acid (RDS) were decreased by O
3 exposure. The O
3-induced reduction of
PN, ascorbic acid content, and its RDS were ameliorated by C
800. Salicylic acid ameliorated the O
3-inhibition of net photosynthetic rate (
PN) slightly, and O
3-induced depletion of total ascorbic acid and RDS substantially. SA did not increase
PN in non-treated leaves (O
0 plants). However, O
3 exposure elevated the level of endogenous SA. These results show that SA plays a vital role in the defense response to acute O
3 exposure in paddy rice. Effects of SA on O
3-inhibition of PSII and ascorbic acid content were unaffected by elevated CO
2 (C
800).
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