The effect of coherent light on the proliferation of resting and mitogen (PHA) transformed T-lymphocytes was investigated
in vitro. T-cells were separated from human peripheral blood by Ficoll-Isopaque and rosetting techniques. Mitogen- and non-mitogen pulsed lymphocytes were irradiated using the following energy densities: 1.2, 3.6, 6.0, 8.4,10.8, and 13.2 J/cm
2. The wavelength (820 nm) and pulsing frequency (5000 Hz) were kept constant as was the power output (50 mW). Controls were sham-irradiated. Following 3 days of incubation, cell proliferation was assessed using
3H-thymidine incorporation. There was a significant stimulation of lymphocyte proliferation following exposure to 1.2 and 3.6 J/cm
2 in the group not treated with PHA. However, the higher energy densities of 10.8 and 13.2 J/cm
2 were inhibitory to T-cell proliferation. Laser irradiation following mitogen addition to T-lymphocytes had an inhibitory effect on lymphocyte proliferation in all the groups studied. The results obtained suggest that the responsiveness of T-lymphocytes to laser therapy varies according to: (1) the amplitude of energy density, and (2) the status of T-cell activation, only cells unstimulated by mitogen being capable of a positive response.
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