Macrophage-like U-937 cells were exposed in vitro to noncoherent 660 nm wavelength light produced by a superluminous diode at a power density of 120 mW/cm2 over a range of energy densities from 2.4-9.6 j/cm2. Twelve hours after irradiation, the medium in which the macrophages. had been growing and which contained growth factors secreted by them was added to 3T3 fibroblasts, also growing in in vitro. and its, effect on fibroblast proliferation assessed It was found that by 96 h after addition of the macrophage-conditioned medium there was a statistically significant increase in fibroblast proliferation in the cells exposed lo supernatants from sham-irradlatet1 macrophages. The most effective energy density was found to be 7.2J/cm2 and the least effective 9.6 J/cm2.
Non-union and delayed union of long bone presents a formidable prospect to the patient. Low reactive-level laser therapy (LLLT) has. been shown to be effective in activation of osseous regeneration. The patient with delayed union was treated with a diode laser emitting at a nominal 890 nm. Complete healing of delayed union with remodeling was achieved.
Lasers have been applied in many aspects of medicine. In this report. the cytogenetic effects of' low power laser irradiation on the cultured human peripheral lymphocytes were studied, The lasers used are argon. He-Ne and GaAlAs diode lasers in low power density. The mitotic index, chromosomal aberrations (including chromosome or chromatid breaks and gaps) und scores for sister chromatid exchange of lymphocytes were observed. Our experiments showed that irradiation with argon laser at the dose of 30-300 J/cm2 has dose-related cytostatic effects, Mitosis was inhibited completely when dose was 240 J/cm or over, There was a significant increase in the frequency of chromosome and chrematid breaks and gaps (p <0.05 with paired t-test) and also an increase of sister chromatid cxchange after 180 J/cm2 (p < 0.05 with paired t-Test). As to the other lasers, there were no significant increase in the frequency of chromosome aberrations. There was an increase of mitosis after GaAlAs diode (0.5 to 1.5 J/cm2) and He-Ne laser (1 to 4J/cm2) irradiation. however, it was not significant statistically.
In this paper. 50 patients suffering from female tubat infertility had received the therapy of CO2 and He-Ne laser acupoint irradiation. The acupoints of 'Uterus' and 'Baliao' werw selected for laser irradiation. A course of therapy consisted of 25 irradiations. After finishing 1-2 courses of treatment. 26 women conceived within 12 months. It is our belief that the laser acupoint irradiation's promotion of blood circulation and dispersion of stagnant fluids might be an important factor in relieving sterility in these cases.
This paper presents the results of a study on soft tissue injury treated with laser therapy. Sixty patients (40 males and 20 females. whose ages range 32-68 years) with sacrospinal muscle strain, external humeral epicondylitis, or lumbar muscle strain were selected for this study. After laserr therapy was received in these patients. their condition all bud an improvement immediately The results showed that the rate of recovery, very good, and good were 50%, 31.7%, and 15% respectively. We believe that the laser therapy for some soft tissue injury is an effective treatment.
Recently, there has been an increasing amount of interest in the application of low reactive level laser therapy (LLLT) for pain attenuation or pain removal. However, the mechanisms by which LLLI- works are not yet full understood. We measured the phagocytic rate using the luminol-dependent chemiluminescence method and a Neuroprobe 48-cell chemotaxis chamber to examine the chemical dependent motion of neutrophils with and without LLLT irradiation. This study showed that at a 15-s exposure, 15, 30, 45, and 60mW output pewers (0.225, 0.45, 0.678 and 0.9 J) shortened the peak time compared with control; peak height increased at all four output powers; and neutrophil random motion count also increased across the board. At a 30-s exposure, doses of 0.45, 0.9, 1.356 and 1.8 J caused no significant shortening of peak time compared with control, but the peak height increased significantly for output powers of 15, 30 and 45 mW. Neutrphil random motion increased for the dose of 0.45 J (15 mW). For a 60-s exposure at the same output powers as above. doses of 0.9, 1.8, 2.7 and 3.6 J respectively, neither peak time nor peak height nor neutrophil random motion count showcd any significant difference compared with the controls.