Stem cell transplantation has attracted attention as an alternative to liver transplantation. Side population (SP) cells are among the most promising candidate transplant cells. SP cells are capable of excreting the DNA-binding dye Hoechst 33342, and are found in various organs. In a previous study, we found that among all organs, the spleen was the largest reservoir of SP cells. Because the timing of transplantation is important for effective treatment, temporary storage of cells is often necessary. In this study, we focused on spleen SP cells, and assessed their usefulness as transplant cells by evaluating whether they can be stored by freezing for extended periods. First, rat spleen cells were frozen, and the presence rate of SP cells was measured at each time point. SP cells and non-SP (main population, MP) cells were frozen and the survival rates of the SP and MP cells were compared at each time point. It was concluded that SP cells were resistant to freezing stress, which is an advantageous property for cells used in stem cell transplantation.
The erector spinae muscles are responsible for initiating and controlling all vertebral column movements; however, they may atrophy after lumbar fusion surgery, and their post-surgical muscle fiber composition in adult spinal deformity (ASD) patients is unclear. It is important to assess this composition to strengthen the muscle effectively during treatment.
We aimed to evaluate differences in erector spinae muscles between healthy adults and ASD patients after lumbar fusion surgery using surface electromyographic (EMG) power spectral analysis.
Thirteen women with post-operative ASD and 7 age-matched healthy women underwent the unsupported trunk holding test combined with surface EMG power spectral analysis until exhaustion. The results of the EMG power spectral analysis were compared between the two groups.
The maximum voluntary contractions were significantly lower in the ASD group than in the control group. No significant differences in the initial median frequency (MF) and MF slopes were found between the two groups.
The results suggested that the relative area of each erector spinae muscle fiber in the postoperative ASD patients did not change despite decreasing muscle strength. Future studies need to focus on methods to strengthen muscle fibers to improve outcomes.
Pica is the persistent behavior of eating non-nutritive and non-food substances, classified as an eating disorder and often found in patients with mental retardation. Diagnosis is difficult in cases where there is no sighting information.
An 11-year-old girl with intellectual disability and a history of pica who complained of intermittent abdominal pain with non-biliary vomiting was referred to our hospital. The patient showed abdominal distension, and an abdominal mass was palpable in her upper abdomen. An enlarged stomach was observed on X-ray examination, and a foreign body in the stomach was suspected on computed tomography. Upper gastrointestinal endoscopic examination revealed a mass of denatured vinyl gloves occupying the distended stomach. Percutaneous endoscopic intragastric surgery was performed to avoid gastrointestinal injury by removing the denatured vinyl gloves with sharp edges.
A 29-year-old man with a history of two open heart aortic valve surgeries presented with symptomatic heart failure secondary to severe paravalvular leakage (PVL) after mechanical aortic valve replacement. PVL was evaluated using transesophageal echocardiography (TEE) and computed tomography. Based on preprocedural imaging, the PVL was a 7 mm × 3 mm oval defect between the mechanical aortic valve and the annulus. We implanted two Amplatzer vascular plug II (Abbott Laboratories, Chicago, USA) (8 mm) side by side through the bilateral femoral arteries under fluoroscopic and TEE guidance. Aortic regurgitation was significantly reduced immediately post-implantation, and no procedural complications were observed. The patient’s heart failure symptoms resolved a year later, and he showed no hemolysis. We could successfully treat a PVL with transcatheter closure.