Flow injection analysis (FIA) method is a chemical analysis method in which a sample is introduced into a carrier solution flowing continuously, mixed and reacted with another continuously flowing reagent solution, and the reaction products are detected by a detector downstream. Sequential Injection Analysis (SIA) is an analytical method that uses a computer-controlled syringe pump and multi-port valve to sequentially inject sample and reagent solutions into a holding coil where mixing and chemical reactions are performed by bidirectional flow, and the reaction products are delivered to a detector for measurement. The ion-selective electrode (ISE) method is an electrochemical analysis method with extremely high selectivity for the target ions to be determined, and is useful as a detector in flow analysis such as the FIA and SIA methods. Several reviews on the use of electrochemical detectors including ISEs in flow analysis methods such as FIA and SIA have already been published. However, they mainly deal with those based on amperometry and voltammetry. The applications of ISE detectors to flow analysis using enzymes and enzyme-linked immunosorbents (ELISA) are rarely covered in these articles. Therefore, this mini-review describes the use of ISEs in flow analysis using enzymes and ELISA.
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