Two strains of oval-rod shaped, Gram-negative, phototrophic, purple non-sulfur bacteria designated JA580
T and JA595 were isolated from a sediment sample collected from a brown pond. Strain JA580
T was designated as the type strain, while strain JA595 as an additional strain has similar characteristics to the type strain. Strain JA580
T was non-motile and grew photoheterotrophically with a number of organic compounds serving as carbon source/electron donor. Intracellular photosynthetic membranes were of the vesicular type. Bacteriochlorophyll
a and carotenoids of the spheroidene series were present as the major photosynthetic pigments. Strain JA580
T requires yeast extract for growth. Strain JA580
T has an obligate requirement for sulfide or thiosulfate for growth. C
18:1ω7c, C
18:0, C
18:1ω9c were the predominant components of cellular fatty acids. Phylogenetic analysis on the basis of 16S rRNA gene sequences showed that strain JA580
T clustered with species of the genus
Rhodovulum of the family
Rhodobacteraceae and is most closely related to the type strains of
Rhodovulum adriaticum, Rhodovulum iodosum (96.5%),
Rhodovulum robiginosum (96%),
Rhodovulum imhoffii (95.6%) and other members of the genus
Rhodovulum (<95%). On the basis of phenotypic and molecular genetic evidence, it is proposed that strain JA580
T should be classified as a novel species of the genus
Rhodovulum of the family
Rhodobacteraceae, with the species name
Rhodovulum phaeolacus sp. nov. The type strain of the species is JA580
T (=NBRC 107612
T =KCTC 5963
T).
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