Journal of Insect Biotechnology and Sericology
Online ISSN : 1884-7978
Print ISSN : 1346-8073
ISSN-L : 1346-8073
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  • Justine Bennette H. Millado, Rina Hamajima, Waka Sugiura, Shizuka Mak ...
    2021 Volume 90 Issue 2 Pages 2_021-2_032
    Published: 2021
    Released: August 06, 2021
    JOURNAL FREE ACCESS

     The tumor suppressor protein p53 serves as a crucial mediator of apoptosis induction and is negatively regulated by various upstream cellular factors through post-translational modifications, including ubiquitination. Here, we identified and characterized the coding region of Bombyx mori homologs of bonus, mdm2, sce, and synoviolin with E3 ubiquitin ligase activity, and rad6 with E2 ubiquitin conjugating enzyme activity, which negatively regulate p53 levels and contribute to p53-mediated apoptosis induction in Drosophila melanogaster and/or mammals. Among the characterized B. mori homologs, Bm-p53 (B. mori homolog of p53) responded to BmMdm2 alone. RNAi-mediated knockdown of bm-mdm2 expression caused increased cellular Bm-p53 levels, whereas transient overexpression of Bm-Mdm2 resulted in reduced cellular Bm-p53 levels, indicating that BmMdm2 functions as a negative regulator of Bm-p53 in B. mori cells. Despite considerable increase in Bm-p53 levels after RNAi-mediated knockdown of bm-mdm2 expression, apoptosis induction or caspase activation was undetectable in B. mori cells under the experimental conditions used. In contrast, transient co-overexpression of Bm-p53 and Bm-Mdm2 attenuated Bm-p53-induced apoptosis and effector caspase activity of B. mori cells without an appreciable reduction in Bm-p53 levels that are expressed by corresponding transfected plasmids. These results indicate that Bm-Mdm2 is the prime negative regulator for Bm-p53 involved in apoptosis induction of B. mori cells.

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  • Tsuguru Fujii, Takashi Kiuchi, Takaaki Daimon, Katsuhiko Ito, Susumu K ...
    2021 Volume 90 Issue 2 Pages 2_033-2_040
    Published: 2021
    Released: August 06, 2021
    JOURNAL FREE ACCESS

     We constructed interspecific chromosome substituted strains using the wild silkworm, Bombyx mandarina and the domesticated silkworm, B. mori. In each developed strain, the entire diploid genome, except one targeted chromosome of B. mandarina, was substituted with B. mori chromosomes. We named these semiconsomic strains as T02 to T28, in which each strain number corresponds to the targeted chromosome number. In each of these strains, the target chromosome is heterozygous to that of B. mori. There are many phenotypic differences between these two species. Comparing the phenotypes of each strain enabled us to identify those chromosomes of B. mandarina that influenced some of its traits. We found five B. mandarina chromosomes that were correlated with the dominant phenotypes. The sibcrossing of the T02-T28 strains resulted in the identification of four B. mandarina chromosomes corresponding to the recessive phenotypes.

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