Cryptomeria japonica D. Don is one of the most commercially important conifers in Japan. However, the allergic disease caused by its pollen is a severe public health problem in Japan. A recent nationwide epidemiological survey found that at least 25% of the Japanese population suffers from pollinosis due to pollen of
C. japonica. The primordia of male strobili of
C. japonica develop from June to August and first become visible from July to September under natural conditions. The development of strobili can also be initiated by treatment with gibberellic acid (GA
3). The promotion of flower formation by exogenous GA
3 occurs even in one-year-old seedlings in spite of the fact that formation of strobili usually requires around 20 years after germination under natural conditions. However, development of male strobili and pollen has not been clarified. The Forestry and Forest Products Research Institute has succeeded in the large-scale collection of 10,463 nonredundant full-length enriched cDNA clones of
C. japonica male strobili. The cDNA resources contain homologs of stamen- and pollen-specific transcripts of
Arabidopsis, and provide new information about potential allergens, putative transcription factors including MADS-box genes and floral meristem identity genes, and male sterility genes in
C. japonica. These genes are valuable tools to clarify the development of male reproductive organs. I here introduce a direction of studies on the formation of male strobili, the suppression of formation of male strobili by inhibitors of gibberellin biosynthesis, and flowering control and introduction of male sterility by genetic engineering.
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