After screening 408 crude drugs, we found that
Rizoma Arisaematis increased the cell numbers of mouse blastocysts developed from
in vivo zygotes. We examined the effects of
Rizoma Arisaematis on the
in vitro development of mouse zygotes and embryos produced by ICSI and SCNT, as well as on fetal development. Mouse zygotes were cultured in media containing a water-soluble extract of
Rizoma Arisaematis at various concentrations, and the potential of zygotes to develop into blastocysts and the cell numbers of blastocysts were examined. In addition, the effects of
Rizoma Arisaematis on the
in vitro and
in vivo developmental potential of embryos produced by ICSI and nuclear transfer were examined.
In vitro treatment of zygotes with
Rizoma Arisaematis increased the cell numbers of blastocysts. The proportions of the blastocysts that implanted and developed into fetuses were slightly higher in the blastocysts which were developed from zygotes treated with
Rizoma Arisaematis than those of the control. The
Rizoma Arisaematis treatment of mouse fetal fibroblast cells or embryos produced by ICSI and SCNT did not affect the growth of the cells, or the
in vitro development of the zygotes. The present study demonstrated that
Rizoma Arisaematis improved the quality of embryos developed from
in-vivo zygotes, but not that of embryos produced by ICSI and nuclear transfer.
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