This review focuses on researches regarding separation sciences utilizing the specific properties of microscopic separation fields. One of the topics is related to the separation by capillary electrophoresis (CE). Despite of its superior performances, CE still has major problems to be overcome such as non-specific interactions between capillary surfaces and samples, difficulty in fabricating stationary phases inside micrometer-sized capillaries, and its lower sensitivity. Therefore, in this first section, to resolve these problems, our obtained knowledge and developed methods were introduced. By our obtained knowledge and developed methods, following separations were attained; (i) the charge/size separation of cationic particles as cationic species by CE, (ii) an easy fabrication method of stationary phases in micrometer-sized capillaries, which enables high reproducible chiral separations in packed capillary electrochromatography, and (iii) high selective separation and high sensitive detection of proteins with multi-functional particles. In the second section, “Lipid Nanotechnology” for separation sciences was mentioned. “Lipid nanotechnology” is a technique utilizing superior properties of lipid self-assembled structures for separation. For establishment of separation methods with lipid nanotechnology, characterization methods for separation field, lipid self-assembled structures were described. Then, some our results of separation with lipid nanotechnology were introduced, especially emergence of chiral recognition in lipid bilayer by polymerization, the application of lipid self-assembled structures for flow separation systems, and membrane proteins separation in lipid nano-membrane.
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