Glucose has been reported to reduce the expression of L-type amino acid transporter 1 (LAT1) protein in C2C12 myocytes. We therefore hypothesized that increased dietary carbohydrate and energy intake would reduce LAT1 protein expression in rodent skeletal muscle. Here, we tested this hypothesis. In Experiment 1, to examine the effects of dietary carbohydrate intake, male Sprague Dawley (SD) rats were divided into low-carbohydrate (low-CHO) and high-carbohydrate (high-CHO) diet groups. Each group was fed a low-CHO (20% carbohydrate) or high-CHO (70% carbohydrate) diet, respectively. Total energy intakes of both groups were matched by pair feeding. In Experiment 2, to examine the effects of dietary energy intake, rats were divided into low-energy diet (fed 68% of ad libitum energy intake) and high-energy diet (ad libitum) groups. After 7 days of dietary manipulation, the lower leg muscles on one side were percutaneously stimulated and subjected to one acute bout of resistance exercise. The contralateral leg muscle served as an internal control. We collected gastrocnemius muscle 6 h after contraction. In both Experiments 1 and 2, when results were analyzed by two-way analysis of variance, no main effect of diet on LAT1 protein concentration was observed. Also, there was no main effect of resistance exercise, or no interaction between diet and exercise. These results do not support our hypothesis that increased dietary carbohydrate and energy intake reduce LAT1 protein expression in rodent skeletal muscle. Furthermore, it was shown that diet may not affect the effects of resistance exercise on LAT1 protein expression.

This study aimed to prospectively record the occurrence of adverse events and near-misses during sports in middle-aged and older adults who voluntarily engaged in sports in the community and to clarify the frequency and characteristics of such events. Participants were comprised of 14 groups (14 sports) of middle-aged and older adults who voluntarily played sports in Sakae Ward, Yokohama. The follow-up period was 14 months (November 1, 2022, to December 31, 2023). Representatives of the target groups reported once a month to Sakae Ward via postcards regarding the status of their activities and the occurrence of adverse events and near-misses. The survey items included the age of the main participants in each group, number of activities during the month, duration of each activity, number of participants per activity, adverse events, and near-misses during sports. During the 14-month observation, eight adverse events and two near-misses were reported in the 14 groups, mainly comprising middle-aged and older adults. Of the eight adverse events, four were falls, all occurring in those aged ≥70 years and triggered by environmental problems. In this study, there were only two near-misses compared to eight adverse events, suggesting that the participants may have been unaware of near-misses. Therefore, even for sports played by groups of residents, it is important to identify risks by assessing health conditions prior to exercise and to implement initiatives to foster safety awareness among participants, such as hazard prediction training.

Sleep and nutrition play a crucial role for athletes, contributing to the quality of recovery, optimization of performance, as well as the preservation of health and injury prevention. This study aimed to explore the potential relationships between sleep quality, nutritional status, and dietary habits among Moroccan male professional football players during the competitive period, while examining the influence of sleep quality on nutritional behaviors. This descriptive research included 49 professional male football players. Sleep quality was measured using the Pittsburgh Sleep Quality Index (PSQI) to differentiate “good sleep” and “poor sleep” groups. Dietary intake was collected using the 24-hour recall and analyzed by validated nutritional software to obtain data on energy intake and dietary frequency. The results revealed that 36 participants were classified as having “Good Sleep” quality and 13 as “Poor Sleep” quality. With a significant difference (4.0 ± 0.2 vs. 6.9 ± 0.9, p < 0.001), consecutively. The correlation between the PSQI score and nutritional status variables has revealed significant associations: a negative correlation with total energy intake (TEI) (r = -0.687, p < 0.01), carbohydrate intake (CHO g/kg BM) (r = -0.499, p < 0.01); and well as (CHO%) and (PRO%) ratios (r = -0.294, p < 0.05), (r = -0.292, p < 0.05) consecutively. On the other side, the PSQI score demonstrates a positive correlation with (FAT%) and Fats and butter. These results suggest an association between sleep quality and dietary intake, indicating a potential influence of sleep quality on dietary choices.