Journal of Reproduction and Development
4,085 registered articles
(updated on May 21, 2024)
Online ISSN : 1348-4400
Print ISSN : 0916-8818
ISSN-L : 0916-8818
JOURNAL PEER REVIEWED OPEN ACCESS ADVANCE PUBLICATION
DOAJ Scopus Pubmed
Featured article
Volume 70 (2024) Issue 2 Pages 82-95
Efficient derivation of embryonic stem cells and primordial germ cell-like cells in cattle Read more
Editor's pick

Cover Story:
The process of inducing the germ cell lineage from pluripotent stem cells, referred to as in vitro gametogenesis, aids in comprehending the mechanisms involved in germ cell differentiation and offers an alternative source of gametes for reproduction. Shirasawa et al. have developed a novel method for robust induction of primordial germ cell-like cells (PGCLCs) from newly established bovine embryonic stem (bES) cells (Shirasawa et al.: Efficient derivation of embryonic stem cells and primordial germ cell-like cells in cattle, pp. 82–95). After a 24-hour culture with bone morphogenetic protein 4 (BMP4), followed by a three-dimensional culture with BMP4 and chemicals modulating WNT signaling, bES cells exhibited positive expression for a set of primordial germ cell (PGC) markers, including PRDM1/BLIMP1, TFAP2C, and SOX17. These outcomes are anticipated to have practical implications for the development of stem cell-based reproductive technologies in cattle.

Volume 70 (2024) Issue 1 Pages 1-9
Improvements in in vitro spermatogenesis: oxygen concentration, antioxidants, tissue-form design, and space control Read more
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Cover Story:
For the past century, achieving in vitro spermatogenesis has remained a difficult challenge for researchers. In 2011, Ogawa et al. successfully demonstrated in vitro spermatogenesis in mice using an organ culture method. However, extending this method to other species posed challenges for over a decade. In 2023, Ogawa’s team achieved in vitro spermatogenesis in rats by incorporating several critical modifications to enhance their original technique. This review presents a detailed analysis by Ogawa et al. comparing their method with natural in vivo conditions and other synthetic alternatives (Ogawa et al. Improvements in in vitro spermatogenesis: oxygen concentration, antioxidants, tissue-form design, and space control, pp. 1–9). They systematically explore the merits, limitations, and inherent constraints of the organ culture approach, delving into the specifics of medium composition, the principles of the gas-liquid interphase method, use of microfluidic devices, and innovation of the PDMS-ceiling method. Highlighting the challenges faced, including regulating oxygen concentration, managing tissue formation, and regulating culture space-control. The insights and novel concepts shared in this review are particularly valuable for those involved in culture or related disciplines, providing innovative content, and encouraging further exploration in this field.

Volume 69 (2023) Issue 6 Pages 308-316
Fluctuation of CD9/SOX2-positive cell populations during the turnover of GH- and TSH-producing cells in the adult anterior pituitary gland Read more
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Cover Story:
Cluster of differentiation 9 (CD9) and sex-determining region Y-box 2 (SOX2) positive cells are stem/progenitor cells for hormone-producing cells in the anterior lobe (AL) of the rat pituitary gland. They are located in the marginal cell layer (MCL) facing the Rathke’s cleft between the AL and intermediate lobe (IL) and the parenchyma of the AL. Horiguchi et al. reported that CD9/SOX2-positive stem cells in the AL-side MCL have potential to supply growth hormone (GH) cells when the increase in GH cell population is required, and the AL parenchyma cells may respond to the demand of thyroid-stimulating hormone (TSH) cell supply in the adult pituitary. (Horiguchi et al. Fluctuation of CD9/SOX2-positive cell population during turnover of GH- and TSH-producing cells in adult anterior pituitary gland, pp. 308–316). These findings may provide one of the mechanisms by which hormone producing cells form from adult stem cells in the pituitary.

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