Japanese Journal of Lactic Acid Bacteria

Online ISSN : 2186-5833
Print ISSN : 1343-327X
ISSN-L : 1343-327X

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Volume 24, Issue 3

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Review

Functional genome analysis of unculturable microbial species as genetic resources

Yuichi Hongoh

2013 Volume 24 Issue 3 Pages 167-173
Published: November 10, 2013
Released on J-STAGE: May 21, 2015

DOIhttps://doi.org/10.4109/jslab.24.167

JOURNAL FREE ACCESS

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The majority of microorganisms on Earth are unculturable and their functions are almost unknown. Whereas metagenomics has enabled us to comprehensively capture genome fragments in an unculturable microbial community, the functions of individual members of the microbiota remain unidentified. Recently, an innovative technology, whole genome amplification, has emerged, and now one can obtain the complete genome of an uncultured bacterial species from a small number of cells. Furthermore, even from a single bacterial cell, one can reconstruct a draft genome by single cell genomics. In this review, I introduce the methodology to analyze the genome of uncultured microbes, using termite gut bacterial community as an example. I also outline the current status of single cell genomics and describe perspectives for exploiting unculturable microbes as genetic resources.

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