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Takashi Yaguchi, Junko Ito, Yoshikazu Horie, Reiko Tanaka, Tetsuhiro M ...
Session ID: P1(SI-1)
Published: 2006
Released on J-STAGE: September 12, 2006
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Takashi Yaguchi, Yoshikazu Horie, Tetsuhiro Matsuzawa, Reiko Tanaka
Session ID: P2
Published: 2006
Released on J-STAGE: September 12, 2006
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Tetsuhiro Matuszawa, Takashi Yaguchi, Yoshikazu Horie, Kazuko Nishimur ...
Session ID: P3
Published: 2006
Released on J-STAGE: September 12, 2006
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Purpose
The genus
Emericella is one of the teleomorphs of
Aspergillus. The species are of medical importance as infective pathogens and carcinogenic producers. They are classified based on the surface structure of ascospores by scanning electron microscopy imaging. On the other hand, phylogenetic systematics in the genus is not fully investigated. In this study, we carry out phylogenetic analysis and clarify a relationship between the molecular phylogenetics and morphological knowledge.
Method
The nucleotide sequences were determined on three genes, β - tubulin, rodlet A and internal transcribed spacer (ITS) region in rRNA gene. And dendrograms were described with NJ methods.
Results and Discussion
The dendrograms based on β - tubulin and rodlet A genes are almost corresponding. In the ITS region usually used to specify the species, the homologies among the species are too high to do cluster analysis. Several species having ascospores of smooth convex surfaces with narrow equatorial crests formed a cluster. Other species having ascospores of cancellous, ribbed or appendaged surfaces with narrow crests and smooth surfaces with broad crests formed four different clusters, respectively. Thus, it is revealed that morphological features of ascospores are related with molecular phylogenetics.
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Akiko Takayama, Eiko Nakagawa Itano, Ayako Sano, Mario Augusto Ono, Ky ...
Session ID: P4
Published: 2006
Released on J-STAGE: September 12, 2006
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Koji YOKOYAMA, Li WANG, Swarajit BISWAS
Session ID: P5
Published: 2006
Released on J-STAGE: September 12, 2006
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The cytochrome b gene of a mitochondria code the subunit of electron transfer system enzyme. This protein played the important role of ATP production and exists common to the eucaryotic organism which has a mitochondria. Therefore, cytochrome b protein hold a function, and carries out the code of it has evolved in the functional pressure. A mitochondrial gene is succeeded by cytoplasmic heredity and recombination is hardly caused. Furthermore, since it has two or more gene sets per cell, detection sensitivity is high. It is expectable as a molecular clock of evolution from these reasons. For evolution of a fungus, a fossil is hardly useful except for the very rare case where it petrifies, with a host. Then, using the standard curve was created with the branch age and replacement the rate of amino acid substitution and the rate of base exchange of the eucaryotic organism of each living thing, the age is presumed to be with the fossil, and its cytochrome b gene, and the analysis of the evolution of the pathogenic fungi was tried.Schizosaccharomyces which is division yeast, this genus has 3 species, branch most early in fungi. These species formed same cluster. Ascomycota was divided from the cluster containing ascomycetous yeast, basiodiomycetous yeast, Basidiomycota and Zygomycota. It is clear that only Ascomycota has much one amino acid in analyzed sequence with in eukaryotic organisms.
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Yohko Yamada, Koichi Makimura, Takashi Sugita, Masako Osumi, Hideyo Ya ...
Session ID: P6
Published: 2006
Released on J-STAGE: September 12, 2006
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Kayoko Takizawa, Kazutaka Fukushima
Session ID: P7
Published: 2006
Released on J-STAGE: September 12, 2006
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Kazutaka Fukushima, Kayoko Takizawa
Session ID: P8
Published: 2006
Released on J-STAGE: September 12, 2006
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Seiji Watanabe, Masako Kawasaki, Takashi Mochizuki, Kazushi Anzawa, Hi ...
Session ID: P9
Published: 2006
Released on J-STAGE: September 12, 2006
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Shoko KUWA, Kenjirou NAKAMURA, Shigeji AOKI
Session ID: P10(SI-2)
Published: 2006
Released on J-STAGE: September 12, 2006
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Reiko Tanaka, Junko Ito, Hiroji Chibana, Yuzuru Mikami, Kazuko Nishimu ...
Session ID: P11
Published: 2006
Released on J-STAGE: September 12, 2006
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Masako KAWASAKI, Kazushi ANZAWA, Hiroshi ISHIZAKI, Takashi MOCHIZUKI
Session ID: P12
Published: 2006
Released on J-STAGE: September 12, 2006
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Hiroshi ISHIZAKI, Masako KAWASAKI, Kazushi ANZAWA, Takashi MOCHIZUKI, ...
Session ID: P13(SI-3)
Published: 2006
Released on J-STAGE: September 12, 2006
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Junji Ito, Ayako Sano, Katsuhiko Kamei, Kazuko Nishimura, Makoto Miyaj ...
Session ID: P14
Published: 2006
Released on J-STAGE: September 12, 2006
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Li WANG, Yan-qiu YANG, Dan HE, Liang GUO, Yun-feng ZHANG, Bo ZHANG, Ko ...
Session ID: P15
Published: 2006
Released on J-STAGE: September 12, 2006
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Objective: To investigate the influential factors of SSR-PCR experiments, the optimal reaction system in fungi was established, which was based for the further research on fungal identification and genetic diversity. Methods: The orthogonal design was used to optimize SSR-PCR amplification system of fungi with four factors (Taq DNA polymerase, DNA template, dNTP and primer). The optimal annealing temperature and cycles of SSR-PCR for fungal reaction were proposed. Results: The notable influence of each factor in different levels was found and the quantity of DNA template was the most effective factor. It is 25μL reaction system which contained 1.0U Taq DNA polymerase, 30ng DNA template, 150μmol/L dNTP, 0.25μmol/L Primer. Conclusion: The method of orthogonal design had been invented to solve multi-factor-controlling problems. It is simple, rapid and efficient to get the optimal conditions of PCR which is an effective method deserving to spread.
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Shigeo ENDO, Takashi MIKAWA, Hiromitsu OSAWA
Session ID: P16
Published: 2006
Released on J-STAGE: September 12, 2006
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KEIICHI HAMANO, REIKO TANAKA, KOICHI NISHIGAKI, KAZUKO NISHIMURA
Session ID: P17
Published: 2006
Released on J-STAGE: September 12, 2006
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Hiroki Kawai, Ayako Sano, Hiroyuki Iwahori, Tomihiro Hayakawa, Kazuko ...
Session ID: P18
Published: 2006
Released on J-STAGE: September 12, 2006
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Hideo Takahashi, Kei-ichi Ueda, Kyoko Yarita, Yoshiteru Murata, Eiko N ...
Session ID: P19
Published: 2006
Released on J-STAGE: September 12, 2006
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KISHIO HATAI, AYAKO SANO, KAZUKO NISHIMURA
Session ID: P20
Published: 2006
Released on J-STAGE: September 12, 2006
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Fungal infection due to deuteromycetes occurred in cultured masu salmon (
Oncorhynchus masou) and many fish chronically died. The external and internal clinical signs were swelling of the abdomen and extensive swelling of the posterior kidney. Many pale brown, septate hyphae were found in the kidney. Histopathological examination of the kidney showed large granulomas with the fungal hyphae. The isolated fungus using Sabouraud dextrose agar sympodially produced oblong conidia with 3 septa and had fine verrucose on the conidia. The fungus was classified in a species of the genus
Ochroconis and was compared with
O. tshawytschae reported from chinook salmon (
Oncorhynchus tshawytschae) by Doty and Slater (1946). As they were very similar, we compared with ATCC9915 which is a holotype of
O. tshawytschae and our isolate using scanning electron microscope. As a result, the isolate and ATCC9915 had a kind of verrusose and two kind of verrucose, respectively. And the conidia of the TCC9915 were slender than the isolate in the morphology. Furthermore, color and form of colony were also different.
Molecular characterization of yamame strain based on the D1/D2 regions of LSU rRNA genes was different from that of the known characterization, and showed 80% homology. Yamame strain was included in same cluster with the fungi of the other genus
Ochroconis from cluster analysis for genome. As a result, the yamame strain was thought as a new species in the genus
Ochroconis.
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Masashi Yamaguchi, Misako Ohkusu, Susumu Kawamoto
Session ID: P21
Published: 2006
Released on J-STAGE: September 12, 2006
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Mami Konomi, Masako Osumi
Session ID: P22
Published: 2006
Released on J-STAGE: September 12, 2006
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Masako Osumi, Takahiro Sato
Session ID: P23
Published: 2006
Released on J-STAGE: September 12, 2006
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Haoman Li, Kiminori Shimizu, Susumu Kawamoto
Session ID: P24(SI-4)
Published: 2006
Released on J-STAGE: September 12, 2006
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Kenjiro Takeda, Yingqian Kang, Taichi Hasegawa, Takahisa Kogure, Katsu ...
Session ID: P25(SI-5)
Published: 2006
Released on J-STAGE: September 12, 2006
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Katsukiyo Yazawa, Souji Iida, Taichi Hasegawa, Kennjirou Takeda, Yuzur ...
Session ID: P26
Published: 2006
Released on J-STAGE: September 12, 2006
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Tohru Gonoi, Kenjiro Takeda, Akira Mukai, Katsukiyo Yazawa, Takashi Ya ...
Session ID: P27
Published: 2006
Released on J-STAGE: September 12, 2006
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Moo Kyu Suh, Gyoung Yim Ha
Session ID: P28
Published: 2006
Released on J-STAGE: September 12, 2006
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Background : There have been many studies about concurrent infection with tinea cruris and tinea pedis as a part of dermatophytosis in Korea. However, few studies have been reported about actual percentage regarding the frequency of tinea pedis in patients with tinea cruris and comparison of the etiologic agents in the groin and foot. Objective : The purpose of this study was to investigate the frequency of tinea pedis in patients with tinea cruris and the etiologic agents in the groin and foot. Methods : 189 patients with tinea cruris were examined clinically, frequency of tinea pedis and etiologic agents isolated and identified by cultures on Sabouraud's dextrose agar from September 2000 to August 2005 in Dongguk University Hospital. Results : The ratio of male to female patients was 10.8:1. And most of them were between the twenties and forties. Most (74.6%) of them involved both sites of the groin. Duration of tinea cruris was the most common within one year. The frequency of tinea pedis in patients with tinea cruris was 85.7%. Most of them (69.1%), duration of tinea pedis had longer period than that of tinea cruris. One hundred and thirty nine dermatophytes were isolated from 189 patients with tinea cruris. They were
Trichophyton(T.) rubrum (89.2%),
T. mentagrophytes (7.2%),
Epidermophyton floccosum (3.6%). Of one hundred and sixty two patients with both tinea cruris and tinea pedis, 52 patients (32.1%) had with same species and only four patients (2.5%) had with different species in the groin and foot. Conclusion : Because of the high incidence of concurrent infection with tinea cruris and tinea pedis, we suggest the need of a careful mycological examination for foot in patients with tinea cruris.
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Mizuki Sawada, Sumiko Ishizaki, Haruo Ito, Takashi Harada
Session ID: P29
Published: 2006
Released on J-STAGE: September 12, 2006
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Hideo takenoshita
Session ID: P30
Published: 2006
Released on J-STAGE: September 12, 2006
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Nobuyoshi Hirose, Morio Suganami, Yumi Shiraki, Masataro Hiruma, Shiga ...
Session ID: P31
Published: 2006
Released on J-STAGE: September 12, 2006
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TATSUYA KASAI
Session ID: P32(SII-1)
Published: 2006
Released on J-STAGE: September 12, 2006
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Machiko Fujihiro
Session ID: P33(SII-2)
Published: 2006
Released on J-STAGE: September 12, 2006
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Shigeru Fujita, Takashi Mochizuki
Session ID: P34
Published: 2006
Released on J-STAGE: September 12, 2006
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Hyoe Ogi, Katsutaro Nishimoto, Takashi Mochizuki
Session ID: P35
Published: 2006
Released on J-STAGE: September 12, 2006
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Ichiro Takahashi, Hjime Iizuka, Sawa Otsubo
Session ID: P36(SII-3)
Published: 2006
Released on J-STAGE: September 12, 2006
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Yumiko Kubota
Session ID: P37
Published: 2006
Released on J-STAGE: September 12, 2006
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