To quantify the effect of surface roughness of epiphytic substrates on sessile organisms, the density of oyster larva and gloss unit were measured at three sites of five collectors for spat collection of scallop shells. As a result, higher oyster larva densities were observed at lower gloss units with statistically significant differences. In this study, the surface roughness of greater than 0.25 mm on the grain size of sandpaper was not measured by the gloss unit. The findings demonstrate that it is possible to determine the roughness of the substrate surface where the density of oyster larva is maximum and the gloss unit that indicates it. Through the application of these results, it is expected that oyster larva densities will improve in the future.