Disulfide bond-containing polymer films were fabricated in a radical UV curing process to adsorb metal cations or proteins. Bi-functional methacryl monomers having a disulfide bond were designed and used for the UV curing. Resulting films showed practical pencil-hardness, H to 3H, with 1 J/cm² of irradiation at a wavelength of 365 nm. It was confirmed that disulfide bond-containing films adsorbed colored metal cations such as gold(III) and copper(II) cations. During adsorption experiments, disulfide bond-containing polymer films were not detached from a glass substrate, probably due to relaxation of internal stress by bond exchange reactions between disulfide bonds. Bovine serum albumin seemed to be also adsorbed toward films only in the case that the film had ionic moieties.