On patients who were treated with surgical operation, blood-culture was done to isolate bacteria. In the cases, appearance of bacteriemie was discussed concernning to methods of surgical treatment, time gathered blood for culti-vation, and degree of the disease was judged clinically. 1) Appearance of bacteriemie could not be considered to have any correlation to methods of surgical treatment (in comparison with gingivectomy and flap operation). 2) Detection of bacteriemie was performed most effectively on the blood gathered at 5 minutes of 5 minutes after operation has started was 41.7% operation (41.7%). On the blood gathered immediately after operation tested cases of 25% were considered to have bacteriemie. At 15 minutes after operation, the lowest frequency for isolation of bacteria was obtained (15.2%). As a tendency, bacteriemie were observed frequently at early stage in the surgical treatments. 3) No correlation observed between defection of bacteriemie and state of pyorrhea alveolaris. 4) Most of isolated bacteria were identified as Streptococcus viridans, and others were determined as Staphylococcus.
Most of the studies concerning of periodontal pocket have been made with necropsy or animal materials so far. To investigate vital periodontal pocket, a new approach to biopsy technique has been tried on experimental animals and periodontal patients. This technique is quite limited only for the indications of tooth extraction, for instance malpositioning or hopeless teeth. Using sharp knife, labial gingival specimen is cut off first including the margin of alveolar bone, then tooth is extracted with extreame care. One of each examples of animals and human materials were demonstrated. No serious hazard has been experienced. This technique will be employed for both of basic and clinical investigation.
In the oral examination of 22 Japanese monkeys (macaca fuscata) living in semi-wild conditions at Iwatayama, Kyoto Pref., a female adult monkey, ten years of age, which has been suffering from advanced periodontal disease was found. These monkeys were fed on mostly with raw vegetable diets during the last seven years. This monkey showed severe, almost generalized gingival inflammation, deep pocket formation and remarkable tooth mobility, and its roentgenogram revealed severe alveolar bone resorption in regions of anterior teeth and first molar teeth. Tooth migration and lack of teeth were also observed. Debris and calculus accumulation was found significantly. As a systemic disorder, tendency of pigment deficiency of hair, skin and eyes was noticed, but it was not serious and may not be related with periodontal disease. Nutritional condition was found to be good. Other monkeys examined had only slight or moderate manifestations of gingivitis and none of them showed bone resorption. In conclusion, it is generally considered that monkeys fed on raw vegetable diets in field do not suffer from advanced periodontal disease, but in this case, there were many complicated local etiologic factors and these might cause svere periodontal destruction in a semi-wild monkey.
The change of gingival color offers an important part in the condition or diagnosis of the periodontal disease. But today, how to express the variety of gingival color is entirely up to personal choice of words. In other words the expressions even for a certain color are different from people to people. The point, therefore, is not clear and can only understood vaguely. I have tried some studies on gingival color with the recent chromatic method—especially with the munsell book of color and spectrophotometer. The results obtained from this study are as follow; 1) The gingiva compared with the munsell book of color. 2.5RP-2.5YR5.5-8/2-6 585nm--525nm 2) The gingiva cut off by gingivectomy measured with spectrophotometer. 585nm--545nm, 10P-5YR5-6.5/0-1.5 3) The acrylic resin measured with spectrophotometer. 595nm-620nm, 2.5R-10R4-6/7-10 4) The gingiva compared with gingival shade (G. S. G) of trial production. Value and Chroma… Uper gingiva<Lower gingiva Hue…Uper gingiva=Lower gingiva 5) Papila, marginal area, attached area, of normal gingiva compared with G. S. G. Value…Marginal area=Attached gingiva>Papillary area. Chroma…Marginal area=Attached gingiva≥Papillary area. Hue…Papillary area=Marginal area=Attached gingiva. 6) Morbid gingiva compared with normal gingiva. Value…Normal gingiva>Gingivitis>Periodontitis Chroma…Gingivitis>Normal gingiva>Periodontitis Hue…Compared with normal gingiva, gingivitis, seems to be inclined to the YR direction, while Periodontitis seems to by inclined to RP direction. 7) What we can examine and tell by using Shade Guide are limited. Shade Guide is considered to be insufficient for the strict analysis of gingival color. 8) I could succeed in the measuring of gingival color by the test use of the color meter of trial production, but there are still some unsolued problems left. My next report will cover the improvements of the color meter and its result in use.
In general understanding, the plasmin has fibrinolytic action, so the blood coagulum is disolved with the existence of plasmin. Also, the plasmin acts not only to the fibrin but to the series of coagulation factors and result is bleeding phenomenon. The bleeding from gingiva is the symptom frequently observed in periodontal diseases. Evaluation of the relationship between plasmin series substances and gingiva of periodontal diseases is usually done by studing fibrinolysis series by means of fibrin plate method. The author performed the experiment with the same means of study but in a modified way. The piece of gingiva was put on the plate instead of using the exudate. The advantage of the present method is that the fibrinolysis activity of the plasmin series substances in gingiva is measured by simply using the biological material, and does not require a complicated method to obtain the extracted solution. In conclusion, the activator was recognized in gingiva of periodontal disease. The gingiva of periodontal disease with the blood upon operation, however, may have the plasmin activity or only the activities of the activator.