日本放線菌学会誌

Original Articles

Application of PCR for Selection of Gram-Positive Bacteria with High DNA G+C Content among New Isolates

Liyan Yu, Yoko Takahashi, Atsuko Matsumoto, Akio Seino, Yuzuru Iwai, S ...

2002 年 16 巻 1 号 p. 1-5
発行日: 2002/06/25
公開日: 2008/03/04

DOIhttps://doi.org/10.3209/saj.16_1

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A PCR condition was established to distinguish strains of Actinobacteria from both Gram-negative bacteria and Gram-positive bacteria with low G+C content of DNA by taking advantage of a stable, specific and common insertion of a 100 bp sequence in the domain III of the 23S ribosomal RNA gene of class Actinobacteria. The PCR was designed to amplify a 380 bp fragment from Actinobacteria and a 270 bp fragment from the above bacteria. PCR-based survey of known strains as well as newly isolated soil bacteria confirmed that this PCR worked well to select Actinobacteria. In the survey it was unexpectedly found that several strains provided both 270 bp and 380 bp amplified fragments (the sequences of these two fragments obtained from Agrococcus jenensis were determined), indicating the presence of at least two copies of the domain III of the 23S rDNA gene.

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A Japanese Encephalitis Virus NS3 Inhibitor Produced by a Streptomyces sp.

Masahiro Hatsu, Masahiro Tanaka, Andi Utama, Hiroyuki Shimizu, Kazuhir ...

2002 年 16 巻 1 号 p. 6-8
発行日: 2002/06/25
公開日: 2008/03/04

DOIhttps://doi.org/10.3209/saj.16_6

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Isolation of Actinomycetes from Live Plants and Evaluation of Antiphytopathogenic Activity of Their Metabolites

Yasuhiro Igarashi, Takako Iida, Tomomitsu Sasaki, Noriko Saito, Ryuji ...

2002 年 16 巻 1 号 p. 9-13
発行日: 2002/06/25
公開日: 2008/03/04

DOIhttps://doi.org/10.3209/saj.16_9

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Actinomycetes were isolated from live plants collected in Toyama and Miyagi prefectures, Japan. Three hundred and ninety eight actinomycete strains were obtained from the leaves, stems and roots of 11 harbaceous and 13 arbor plants of agricultural and wild species. n-Butanol extracts of the fermentation broths of these strains were screened for antiphytopathogenic activity using in vitro antimicrobial and in vivo antiinfective assays. About 10–20% of the extracts showed antagonistic activity against fungal or bacterial plant pathogens. In addition, several extracts suppressed the infection of Alternaria brassicicola TP-F0423, a fungal pathogen to seedlings of Chinese cabbages in vivo.

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Studies on Actinomycetes Isolated from Plant Leaves —New plant growth inhibitors A-79197-2 and -3 from Dacthylosporangium aurantiacum SANK 61299—

Masaaki Kizuka, Ryuzo Enokita, Kayoko Shibata, Yoshihiro Okamoto, Yosh ...

2002 年 16 巻 1 号 p. 14-16
発行日: 2002/06/25
公開日: 2008/03/04

DOIhttps://doi.org/10.3209/saj.16_14

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New plant growth inhibitors, substances A-79197-2 and -3, were found to be produced by strain SANK 61299 isolated from fresh plant leaves. Strain SANK 61299 was identified as Dacthylosporangium aurantiacum. Substances A-79197-2 and -3 were determined to be di- and tri- saccharides of streptol, an active ingredient, respectively. Streptol related compounds have been found as components of plants.

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