Cytological studies of seven species of family Euphaeidae were carried out. Among them three Dysphaea and two Euphaea species were investigated for the first time. All the species investigated possessed n=13 complement which is typical number of the family. C-bands were mostly appeared on terminal regions of all the autosomal bivalents in the seven species. The smallest chromosome (m) bivalents in Bayadera indica and Dysphaea ethela were C-negative, while less amount of Cheterochromatin in D. walli. C-bands were observed on both terminals of m bivalents in D. gloriosa and Euphaea fraseri. In the X chromosomes, terminal C-band on one side appeared in Anisopleura lestoides, B. indica and D. walli and dark C-bands on both terminals in E. fraseri and E. ochracea. Entire C-positive X chromosomes was observed in D. ethela and D. gloriosa. Nucleolar organizer regions (NORs) were observed at chiasmatic ends of either one or both terminals of autosomal bivalents. X chromosome possessed dark NOR on one terminal in A. lestoides, D. gloriosa, D. walli and E. ochracea and entirely NOR rich in B. indica, D. ethela and E. fraseri. The m bivalent possessed NOR on one terminal in D. ethela and D. gloriosa, while it was NOR-negative in B. indica, D. walli, and E. fraseri. In sequence specific staining, variable signals were seen but most of the species showed DAPI bright and CMA3 light regions at terminal ends.
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