The reciprocal translocation t(11;14)(q13;q32) is frequently observed in multiple myeloma (MM) and related disorders. The aim of this study was to demonstrate that intraclonal heterogeneity occurs in t(11;14) itself. Fluorescence in situ hybridization (FISH) analyses of interphase nuclei were performed in 12 patients (10 with MM and 2 with other malignant monoclonal gammopathies) with t(11;14). An LSI IGH/CCND1 dual-color, dual-fusion translocation probe, which showed 2 standard types of signal pattern depending on the breakpoints on chromosome 11, was used for the detection of t(11;14). Although, 11 of 12 patients showed either of the 2 standard patterns, 1 patient showed both standard patterns, suggesting a coexistence of 2 clones with different patterns. Furthermore, all cases had clonal and non-clonal cells with various abnormal FISH signal patterns in addition to those with the standard pattern. These findings indicated that t(11;14) itself is susceptible to modification once it has occurred, followed by intraclonal heterogeneity through an evolutionary process of survival. The present study is helpful in understanding the biology of t(11;14) from the viewpoint of intraclonal heterogeneity.

Pseudocydonia sinensis (Thouin) C.K. Schneid. (Chinese quince), the only species of genus Pseudocydonia in the subfamily Amygdaloideae of Rosaceae, is a tree species, the fruits of which are used as an ingredient in candies, juice, and syrups.

This study was conducted to clarify the karyotype of P. sinensis. We found that this species had small, 2n=34 chromosomes, ranging from 1.0 to 1.8 μm in length, with the chromosome complement expressed as a formula of 2n=34=16m+14sm +2st+2^tst.

Chromosome numbers were determined for 44 taxa of the Leguminosae, divided into 28 genera. For the Mimosoideae subfamily: Tribe Mimoseae, Mimosa pudica (2n=78) and Tribe Ingeae, Albizia julibrissin (2n=26); for the Caesalpinioideae subfamily: Tribe Cassieae, Chamaecrista nomame (2n=16); and for the Papilionoideae subfamily: Tribe Sophoreae, Sophora flavescens (2n=18), Tribe Thermopsideae, Thermopsis lupinoides (2n=18), Tribe Trifolieae, Medicago polymorpha (2n=14), Medicago lupulina (2n=16), Trifolium pratense (2n=14), Trifolium repens (2n=32), Trifolium hybridum (2n=16), Trifolium dubium (2n=30), Tribe Loteae, Lotus corniculatus subsp. japonicus (2n=12), Lotus corniculatus subsp. corniculatus (2n=12, 24), Tribe Fabeae, Vicia sativa subsp. nigra (2n=12), Vicia tetrasperma (2n=14), Vicia hirsuta (2n=14), Vicia amoena (2n=24), Vicia japonica (2n=12), Vicia unijuga (2n=24), Lathyrus japonicus (2n=14), Lathyrus palustris (2n=14, 42), Tribe Galegeae, Astragalus sinicus (2n=16, 32), Astragalus membranaceus (2n=16), Oxytropis campestris (2n=48), Tribe Indigofereae, Indigofera bungeana (2n=16), Tribe Robinieae, Robinia pseudoacacia (2n=22), Amorpha fruticosa (2n=40), Tribe Millettieae, Wisteria brachybotrys (2n=16), Wisteria japonica (2n=16), Tribe Phaseoleae, Rhynchosia volubilis (2n=22), Vigna angularis var. nipponensis(2n=22), Pueraria lobata (2n=22), Canavalia lineata (2n=22), Glycine max subsp. soja (2n=40), Glycine max subsp. max (2n=40), Dumasia truncata (2n=20), Amphicarpaea edgeworthii (2n=22), Tribe Dalbergieae, Aeschynomene indica (2n=40), and Tribe Desmodieae, Kummerowia striata (2n=22), Lespedeza bicolor (2n=22), Lespedeza pilosa (2n=20), Lespedeza cuneata (2n=20), Desmodium paniculatum (2n=22), Hylodesmum podocarpum subsp. oxyphyllum (2n=22).

First chromosome counts were determined for Wisteria japonica (2n=16). New chromosome counts were determined for the following three species: Mimosa pudica (2n=78), Trifolium dubium (2n=30) and Astragalus sinicus (2n=32).