We investigated the invasion status of alien lineage of the Pond loach, Misgurnus anguillicaudatus in central eastern Kagawa Prefecture using genetic and morphological approaches. Genetic analyses based on mitochondrial DNA (mtDNA) revealed the invasion of a Chinese lineage at four of the five sites, while the Japanese lineage was restricted to one of the sites. Morphometric characteristics, including the number of branched soft rays of the dorsal fin and caudal peduncle length, exhibited distinct trends between the two lineages. As alien loaches have been introduced for fish farming, they may have spread along major rivers within the prefecture. Accurate lineage identification in future surveys is crucial for the conservation of the native lineage of M. anguillicaudatus.

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2023年7月に愛媛県松山市内の水田に黄色やオレンジ色，白色ないしクリーム色，帯青色の様々な体色を呈する小型の魚類の生息を認め，このうちの10個体を採集した．これらはその形態からメダカOryzias speciesの観賞用の個体であると判断され，これらのメダカはブリーダーないしはそれに関連する飼育者によって遺棄されたことが疑われた．この事例を通して，メダカの野生個体群に遺伝子汚染を起こさないために，観賞用に繁殖させたメダカの遺棄は慎むべきであることを考察した．

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The Grey-backed Thrush (Turdus hortulorum) was observed in Kochi City, Kochi Prefecture, Japan, in April 2024. This marks the first recorded sighting of this species in Kochi Prefecture.

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We observed a Lesser Frigatebird, Fregata ariel at Nii-Coast, Kochi Prefecture, on 19 October 2023. This is a valuable record of Lesser Frigatebird in Kochi Prefecture.

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Echolocation calls of the Oriental free-tailed bat, Tadarida insignis, were recorded at five sites in the southwestern region of Shikoku, Japan. The pulse duration (D) of the calls averaged 17.5ms, the frequency of maximum energy of the pulse (PF) averaged 13.5kHz, and end frequency (EF) averaged 11.3kHz, start frequency (SF) averaged 18.7 kHz, bandwidth (BW) averaged 7.4kHz, interpulse interval (IPI) averaged 495.3ms. The duration and frequency of pulses obtained for this species in the southwestern Shikoku region did not differ significantly from those reported in the Kyushu and Chugoku, Chubu regions. While some variation in search phase call structure between sites was suggested, the overall results were considered to be continuous.

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