A hydraulic experiment was conducted for quantitative evaluation of the exfoliation dynamics of algae caused by sediment transport. As a result of investigating the relationship between the sediment particle diameter d and the unit width sediment discharge qB with the exfoliation characteristic value p, it was shown that each parameter influences the value of p and that there is a range showing the correlation. It was also shown that the quantity of algae exfoliation can be expected by the amount of work Wx calculated with hydraulic conditions, sediment particle diameter d, and the unit width sediment discharge qB as parameters. Further studies are needed because there are many problems in application in the field, such as examination of the resistance coefficient due to the shape of the bed, and changes in the exfoliation characteristics due to the growth stage of algae.