In this paper, we proposed a device for high effective internalization of magnetically driven nanomachines into a single cell. Carbon nanocoil sputtered Ni is used as a nanomachine. The nanomachine can be driven by rotating magnetic field. In order to increase efficiency of internalization of nanomachine into a cell, we proposed micro square patterns pasted cell non- adhesion polymer on convex regions as a cell culture environment that adhered cells at the bottom of square patterns efficiently take in nanomachines attracted to surface with vertical magnetic field. We succeeded fabrication of 50 μm and 100μm square patterns on a biocompatible material.
In this paper, we proposed an intracellular nanomachine for controlling an organelle and a cell function. Carbon nanocoil sputtered Ni was used as a nanomachine. The nanomachine can be driven by rotating magnetic field. In order to optimize the driving performance, the velocity dependence on the Ni thickness was evaluated and we tried to introduce a nanomachine into a cell. As a result, the maximum velocity of 2.8 μm/s was obtained for Ni thickness of 80 nm. We found that the optimized Ni thickness existed for the nanomachine. Furthermore we succeeded in the introduction of the nanomachines into a cell and driving the nanomachines inside cells. These results will lead to smaller nanomachines to investigate the information inside cells.
東京大学 大学院工学系研究科 特任准教授 安楽 泰孝
In this paper, we report on the intracellular introduction of nanomachines, with the final goal of measuring intracellular physical properties by magnetically driven nanomachines. In this study, a microchip and centrifugal force were used to introduce nanomachines into a red blood cell. The microchip was fabricated with a structure of SU-8 on a slide glass. Micropockets are formed in the structure to hold red blood cells. The size was a square with one side of 10 μm considering the size of a red blood cell. We dropped the solution containing red blood cells and nanomachines into the inside of the structure and cover the structure with a cover glass. Thereafter, the microchip was centrifuged. Due to centrifugal force, red blood cells and nanomachines move toward micropockets, at which time nanomachines are introduced into the red blood cells. As a result of microscopic observation, nanomachine was introduced into red blood cells.