CaNa₂EDTAの静脈注射により作出した腎機能障害牛および腎障害自然発症牛の尿中から低分子蛋白を分離精製した。即ち、これらのウシの濃縮した蛋白陽性尿から塩析法によりグロブリン分画を分離し, これをSephadex G-100カラムを用いたゲル濾過法で5つに分画した. これらのうち, 最小分子量を示した分画をさらにDEAE-celluloseを用いたイオン交換カラムクロマトグラフィーにより2つに分画した. このうちの1分画は, SDS-PAGEにより単一のピークを示し, かつ分子量が12,000と推定され, ヒトβ₂-microglobulin(MW11,800)と類似した分子量であった. 一方, この分画は, セルロースアセテート膜電気泳動法により正常ウシ血清のγ-グロブリン位に移動し, さらに, ゲル内沈降反応において, それに対する抗血清との間に1本の沈降線を形成したが, 抗ヒトβ₂-microglobulinとの間には沈降線が形成されなかった. また, この抗血清と実験的腎障害牛および腎障害自然発症牛の各濃縮尿との間には, 沈降線が形成されたが, 正常牛の濃縮尿およびヒトβ₂-microglobulinとは沈降線を形成しなかった.

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Unkv-rya is a school of Kenjutsu, the Japanese traditional swordsmanship.In the early days of the Edo era, it was established by Itori Kyoun who learned Kenjutsu at the Kô-ryû school and Mujû shinken-ryû school. Kengo Tominaga was the first researcher who referred to the lineage, the old documents, the Kata (forms) and the method of bamboo sword practice of this school in the Kumamoto district. There are few studies on this school except his posthumous works.
The porpose of this study is to clarify the details related to the tradition of Unkô-ryû, through the analysis of the documents and materials we have recently discovered and offered by Mr. Hiromichi Inoue, the present master of this school. The results can be summarized as follows; 1) All the hereditary masters of Unkô-ryû styled themselves with the names which include the same word Un. It was succeeded to the usage of Muju shinken-rya, for example, Harigaya Sekiun or Kodegiri Ichiun.2) In Unkô-ryû, the licence on the first and the middle grades are “Zuijun-no-shô” and “Sankyôroku”, the documents of Kô-ryû. Two licences out of three on the final grade are “Sekiun-rya Kenjutsu-sho” and “Tenshin-dokuro” written by Kodegiri Ichiun, the second master of Muju shinken-ryû.3) The method of bamboo sword practice in this school was also succeeded to the style of Muju-shinken-ryû.-Two persons faced each other try to. do the same technique “Men (head)-striking”, from the same posture “Hassô”, at the same time. This was the only practice called Aiuchi, and a very unique one disregarded victory or defeat. Thus Unkô-ryû is a Kenjutsu school combined these two schools.; however, it is supposed to have been influenced by Muju shinken-ryu much more than by Kô-ryû.

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In recent years, the study of Shinto神道priests on the local level has progressed under the historical concept of "social status periphery".Using this concept, historians of the Tokugawa Period have shown a relative instability in the status of Shinto priests in conparison to Buddhist monks, who played the predominate religios role during the period.In an attempt to further clarify such a line of argument, the present paper looks into the organization of Shinto priests in the province of Aki, a region overwhelmingly dominated by the Buddhist Shin Pure Land Sect.For Shinto priests on the social status periphery, one way of escaping such social instability was to organize under the patroage of such influential families as the Yoshidas吉田 and thus ensure their social position.However, intervention by the Yoshida family in the affairs of local Shinto priests was by no means always welcome.On the occasion of the dispatch of agents to adron the shrines of Hiroshima Feud with heihaku幣帛 symbols of worship, the Yoshida family took the opportunity to install purification deputies(注連没頭)in each district for the purpose of registering local Shinto priests.However, such a policy was ineffective due to a lack of organization among the priests in the region.A similar opportunity was presented in 1804 for the Yoshida family to intervene, designed to influence the region for a long time to come.This took advantage of the holding of ceremonies, called kokuonsai国恩祭, for the first time in Hiroshima Feud.They were events that gathered the Shinto priests of each locality together to play for the health and safty of the feudal lordout of gratitude for his benevolence.Such gatherings helped nurture a sense of solodarity and organization among local priests and thus enabled the Yoshida family's intervention of the early 1880s to have a more lasting effect on the region than in the 1740s.The paper concludes that the act of Shinto ritually repaying the feud for its kindness clearly a conscious attempt they were suffering.

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Few people know two historical and/or archaeological shipwrecks at Tokyo bay. One is Haya-maru located at the southern-side of line drawn between Futtsu Cape at Chiba Pref. and Kannonsaki at Kanagawa pref., the other is the Oneida located at the northern-side of the line. While the former is a national vessel, the latter is a foreign warship (United States). Sinking at internal waters (territorial sea), the latter is generally under control of Japan with the exception of removal of artifacts (UNCLOS article 303 § 2). In the case of Oneiada, United States abandoned its property right by selling. Taking all the shoreline surrounded by the Sea into consideration, Japan must face up to the international trend of UNESCO Convention on the Protection of the Underwater Cultural Heritage (adapted 2001, entry into force 2009, not ratified as to Japan). This article aims to reveal some defects in the Japanese legal system through an examination of the treatment of two shipwrecks at Tokyo Bay. The most important thing is to pay attention to an idea as follows. It would be seen in the citation of titled A Study of Remains conservation Method: Underwater Remains by Monuments and Sites Division of Agency for Cultural Affairs (March, 2000). " To prevent the alteration and free salvage of underwater remains, likewise the method of the protection of remains on land, the points and situations of underwater remains should be firmly grasped, thereby such points and places positively need to be notified to the public as sites which contain buried cultural properties. It could be the first step for the protection of underwater remains above all to compel those who investigate underwater remains or carry out engineering works and so forth to notify and/or report in advance." (pp.73-74). Therefore this article particularly attaches great importance to legal aspects as well as historical stories. Such a narrative nature and background gives rise to archaeology, which could be legal interest perceived as highly beneficial interest to the public as a whole.

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