長年「夢の反応」とされてきたメタンからメタノールの合成に成功したことをきっかけに、大阪大学大学院薬学研究科の

教授らはコンソーシアムを立ち上げ、この酸化反応の活用を進めてきた。さまざまな分野の企業が参画し、応用先は広がりを見せている。タンパク質の立体構造解析に使われる「クライオ電子顕微鏡」の試料調製への応用では世界の注目を集める。「夢の反応」があらゆる産業へ技術革新をもたらそうとしている。

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we proposed a new processing technique for protein crystals, pulsed UV laser soft ablation (PULSA) . Several kinds of protein crystals were successfully processed by laser irradiation at a wavelength of 193 nm. Unchanged resolution limit between pre- and post-processings in X-ray diffraction measurements showed PULSA had little influence on the crystallinity of the non-irradiated section. This technique will be a powerful tool for processing fragile biological-macromolecule crystals and will accelerate protein structural analysis.

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We developed crystallization method of a protein molecule by laser irradiation, and call this process Laser Irradiated GrowtH Technique (LIGHT) . Effective crystallization was confirmed by applying a femtosecond laser. The crystallization period was shortened by LIGHT. Protein crystals were also obtained by LIGHT from normally uncrystallized conditions. These results indicate that laser irradiation generates crystal nuclei; protein crystals can then be grown from the nuclei that act as seeds in a supersaturated solution. We should optimize the laserirradiated condition to control the number of crystals and to obtain high-quality crystals because protein crystallization depends on the conditions.

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