Permeability of meningls to uranin is greatly increased in case of inflammation of meninges. Based on the above mentioned fact, uranin is used to establish diagnosis and prognosis of suppu-rative meningitis.In this experiment, it was planned to decide proper dose, method of injection of uranin and proper time to obtain spinal fluid after the injection.The experiment was carried out using rabbits, half of them being healthy and the other half experimentally infected by pneumococcus type I to iuduce meningitis.
The conclusions were as follows:1.In case of healthy rabbits, the highest peak of the density of uranin in spinal fluid was observed one hour after injection.
Uranin was given intramuscularly or intravenously, and in both cases the highest peak was observed one hour after the injection and the content of uranin was less than 10γ/dl.
2.In case of rabbits suffering from meningitis, the highest peak was also observed one hour after the injection and the average content was 71.6γ/dl by intramuscular injection and 81.6γ/dl by intravenous injection.
According to the statistical analysis, the difference of the level of the content between healt-hy rabbits and sick rabbits was proved significant.Therefore the test is tnought to be a valuable method to diagnose suppurative meningitis.
3.The time to obtain the spinal fluid should be one hour after the injection, because the hig-hest content of uranin in the fluid was observed at this time, both by intramuscular and intrave-nous injection.
4.In case of sick rabbits, the difference between intramuscular injection and intravenous inje-ction was insignificant.
Clinicnlly, in case of intravenous injection side-effects are observed frequently, and pit would be concluded that intramuscular injection is better than intravenous injection.
5.In case of sick rabbits, the difference between in doses of 0.03gram and of 0.06gram per K.G.M.waf insignificant.
Consequently, the sufficient doses of uranin for the test are considered to be 0.03gm.per kgm.

抄録全体を表示