Nachdem wir Kolibazillenantiviru sund Kolibazillenextrakte auf das isolierte überlebende Darmröhrchen der früher immunisierten Kaninehen und Meerschweinchen hatten einwiken lassen, untersuchten wir den Zusammenhang zwischen der Antiviruswirkung und der Anaphylaxie des Darmrohrs unter dem Merkmal des Prätipitintiters und der Bewegung der Darmrohrchens und kamen zum Schluss, dass die Wirkung des Koliantivirus, die Darmbewegung zu fördern und dadurch dessen Tonus zu steigern in keinerlei Zusammenhang mit der Anaphylaxie steht.

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Using the New Yoshida Tumor, the author investigated the energy metabolism by observing the inhibition of the respiration and the inorganic phosphorus intake of the cells by NaF, 2, 4-dinitrophenol (DNP), and aureomycin (AM).
The following results were obtained.
1) By the addition of glucose into the reaction media, the respiration, the respiration of the tumor cells was inhibited to some extent compared to the control, though the inorganic phosphorus intake of that was retained almost same as the control. Whereas the addition of Mg into the media did not show remarkable effects on the respiration and on the phosphorus intake.
2) When glucose was not added into the media, the respiration was markedly inhibited by NaF in dilution of 10^-1 M, but was rather accelerated by that in dilutions of 10^-2 and 10^-3 M: the phosphorus intake was also inhibited by NaF in diluticn of 10^-1 M, and conversely accelerated by that to slight extent in dilutions of 10^-2 and 10^-3 M. However, as the addition of glucose into the media, it was also inhibited by NaF in dilution of 10^-1 M; and conversely accelerated by that to slight extent in dilutions of 10^-2 and 10^-3 M. However, as the addition of glucose into the media, it was noticed that the respiration was inhibited by NaF in any dilutions and that the phosphorus intake was accelerated, especially in dilution of 10^-2 M.
3) The endogeneous respiration and the phosphorus intake of the tumor cells were inhibited by DNP in any dilutions, 10^-3, 10^-4, and 10^-5 M. The inhibitions of these were reduced as the addition of glucose into the media, respectively, especially on the phosphorus intake.
4) AM did not show inhibitory effect so remarkably on the phosphorus intake irrespective of the additicn of glucose; but showed considerable inhibition on the endogenueous repiration in dilutions of 10^-3 and 10^-4 M. The inhibited endogeneous respiration was not recovered by the addition of glucose in contrast with the inhibition by DNP.
5) The O₂ uptake and the phosphorus intake were also studied on the cells. suspended in 0.85% NaCl, ascitic supernatant, and amniotic fluid of chick embryo, respectively, in all reaction media glucose was added as substrate. And both accelerations of the respiration and of the phosphorus intake were remarkable in order of 0.85% NaCl, amniotic fluid and ascitic supernatant. The inhibitory effects on the O₂ uptake and the phosphorus intke by NaF, DNP, and AM were regarded to be similar to each other in respective cell suspensions mentioned above.

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It is well known that in malignant tumors, their glycolysis are marked both under aerobic and anaerobic conditions, and this is the most important biochemical property common to all malignant tumors.
Using the New Yoshida Tumor, the author investigated the quantitative relation in its glycolysis and the effect of thionin on its respiration in order to observe some aspects of its terminal respiration system and besides, the effect of some inhibitors on its glycolysis was studied.
The results were as follows:
1) It was found that as reported up to this time, both under aerobic and anaerobic conditions, the glycolysis by this tumor was as marked as by other sorts of tumors and lactic acid was formed in a great quantity.
2) When glucose was added as the substrate, its endogenous respiration was inhibited and the higher the concentration of glucose was, the more marked the inhibitory effect was and yet, in any low concentration of glucose, its respiration was not accelerated.
3) The respiration inhibited by addition of glucose was recovered by addition of thionin: In this case, however, lactic acid formation was decreased.
4) When T. C. A. cycle intermediates were added as substrates, its respiration was little accelerated, but by addition of thionin at the same time as the intermediates, its respiration was accelerated to sme degree.
Further, the respiration accelerated by addition of thionin was reduced after a long time reaction and conversely inhibited slightly.
5) KCN inhibited its respiration, but had no effect on glycolysis itself at all, whereas monoiodacetic acid, 2, 4-dinitrophenol, aureomycin and NaF inhibibited not only its respiration but its glycolysis at the same time.

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