In this study, I attempted to analyze osteoinductive activity in prefabricated neovascularized bone flap using an experimental model of autologous bone and allogenic freeze-dried bone, in rats.
Wistar rats were divided into two groups according to transplanted bone that is autologous iliac bone and allogenic freeze-dried bone.
All flaps were reharvested at 2, 4, 8, and 12 weeks after transplantation for radiographic, histological, and immunohistochemical examinations.
The results obtained were as follows:
1. In the autologous iliac bone group, osteogenesis was observed at the second week after transplantation. Immunohistochemical expression of osteopontin was observed in new bone matrix.
2. In the allogenic freeze-dried bone group, weak osteoinduction, such as endochondral bone formation, was observed at the fourth week after transplantation. However, transplanted bone chips were absorbed at the twelfth week after transplantation.
3. In the autologous iliac bone group, new bone formation with bone bridges between bone chips was observed around the vascular bundle. The newly formed bone expressed osteocalcin immunohistochemically at the twelfth week after transplantation.
4. The method of prefabricated neovascularized bone flap using autologous iliac bone chips was found to be a useful axial pattern flap with osteoinductive properties, and possibility of clinical application in bone reconstruction was shown.
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