1. TMPD reduces nitrite to nitric oxide through cytochrome c-552 (denitrifying bacteria) in the presence of denitrifying enzyme. The optimal pH is 6. 4-6. 6.
2. Carbon monoxide combines with the denitrifying enzyme itself and inhibits its activity; the inhibition is not relieved by light. There occurs a competition for the denitrifying enzyme between CO and NO produced by the denitrification.
3. Nitric oxide production is strongly inhibited by KCN but not by NaN₃ in the presence of TMPD. Effects of various other inhibitors are also investigated.
4. By these results the possibility that NO may be an intermediate in the denitrifying reaction was reinforced.
5. A possible scheme for the process of denitrification was proposed.

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