The lauryl maltose neopentyl glycol (LMNG)-assisted sample preparation (LASP) method was adapted for the process of on-beads digestion in co-immunoprecipitation with mass spectrometry (coIP-MS). In addition, to improve peptide recovery, the dried peptides were dissolved in 0.01% decyl maltose neopentyl glycol (DMNG) after desalting at the SDB Stage tip. The adapted LASP method and the lysis of dried peptides with DMNG increased the number of proteins identified in coIP-MS.
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