清水 恒平
ジャーナル
フリー
*清水 康平, 山口 雄生, 丸山 泰一, 井手 一郎, 東海林 弘, 石井 久夫
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Miwa Sugiura, Shogo Ogami, Fabrice Rappaport, Alain Boussac
生物物理
2014年
54 巻
supplement1-2 号
S236-
発行日: 2014/08/20
公開日: 2017/05/01
ジャーナル
フリー
Masaru Kato, Hisako Sato, Miwa Sugiura
生物物理
2014年
54 巻
supplement1-2 号
S236-
発行日: 2014/08/20
公開日: 2017/05/01
ジャーナル
フリー
Ryuma Sato, Hirotaka Kitoh-Nishioka, Tsutomu Kawatsu, Kei Yura, Koji Ando, Takahisa Yamato
生物物理
2014年
54 巻
supplement1-2 号
S236-
発行日: 2014/08/20
公開日: 2017/05/01
ジャーナル
フリー
生物物理
2014年
54 巻
supplement1-2 号
S236-
発行日: 2014/08/20
公開日: 2017/05/01
ジャーナル
フリー
Yuki Akiyama, Yusuke Nakasone, Osamu Hisatomi, Yoichi Nakatani, Masahide Terazima
生物物理
2014年
54 巻
supplement1-2 号
S236-
発行日: 2014/08/20
公開日: 2017/05/01
ジャーナル
フリー
Kyosuke Oshima, Arisu Shigeta, Yoshiteru Makino, Izuru Kawamura, Takashi Okitsu, Akimori Wada, Satoru Tuzi, Akira Naito
生物物理
2014年
54 巻
supplement1-2 号
S236-
発行日: 2014/08/20
公開日: 2017/05/01
ジャーナル
フリー
*山口 雄生, 清水 康平, 佐野 大輔, 松崎 厚志, 田中 有弥, 上野 那美, 森澤 勇介, 石井 久夫
会議録・要旨集
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*清水 康平, 深川 弘彦, 森井 克行, 金城 拓海, 佐藤 友哉, 石井 久夫
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*清水 康平, Levine Igal, Zohar Arava, Kulbak Michael, Kahn Antoine, Hodes Gary, Cahen David, 石井 久夫
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*石井 久夫, 木全 俊輔, 井手 一郎, 丸山 泰一, 山口 雄生, 松崎 厚志, 清水 康平, 田中 有弥
会議録・要旨集
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*石井 久夫, 木全 俊輔, 濱田 北斗, 菊池 武文, 井手 一郎, 佐野 大輔, 丸山 泰一, 山口 雄生, 松﨑 厚志, 清水 康平, 田中 有弥
会議録・要旨集
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ジャーナル
フリー
Pulpitis often causes referred pain in opposing teeth. However, the precise mechanism underlying ectopic pain associated with tooth-pulp inflammation remains unclear. We performed the present study to test the hypothesis that functional interactions between satellite glial cells (SGCs) and trigeminal ganglion (TG) neurons are involved in ectopic orofacial pain associated with tooth-pulp inflammation. Digastric muscle electromyograph (D-EMG) activity elicited by administration of capsaicin into the upper second molar pulp (U2) was analyzed to evaluate noxious reflex responses. D-EMG activity was significantly increased in rats with lower first molar (L1) inflammation relative to saline-treated rats. Significantly increased expression of glial fibrillary acid protein (GFAP), a marker of activated glial cells, and connexin 43 (Cx43), a gap-junction protein, was observed in activated SGCs surrounding U2-innervating TG-neurons after L1-pulp inflammation. Daily administration of Gap26, a Cx43-inhibiting mimetic peptide, into the TG significantly suppressed capsaicin-induced D-EMG activity enhancement and reduced the percentage of fluorogold-labeled (U2-innervated) cells that were surrounded by GFAP-immunoreactive (IR) and Cx43-IR cells after L1-pulp inflammation. These findings indicate that tooth-pulp inflammation induces SGC activation and subsequent spread of SGC activation in the TG via Cx43-containing gap junctions. Thus, remote neuron excitability becomes enhanced in the TG following tooth-pulp inflammation, resulting in ectopic tooth-pulp pain in the contralateral tooth.
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A study was conducted to evaluate the mechanisms underlying ectopic orofacial pain associated with tooth pulp inflammation in rats. We observed a significant decrease in the head withdrawal threshold (HWT) response to mechanical and heat stimuli applied to the ipsilateral facial skin upon application of complete Freund’s adjuvant (CFA) to the upper first molar (M1TP) in comparison to application of vehicle. A large number of trigeminal ganglion (TG) neurons showed transient receptor potential vanilloid 1 (TRPV1) immunoreactivity (IR), and some of them were retrogradely labeled with fluorogold injected into the facial skin. A large number of cells showing IR for glial fibrillary acidic protein (GFAP) were observed in the 2nd compared to the 1st or 3rd branch regions of the TG, and TG cells innervating the facial skin were also surrounded by GFAP-IR cells. After administration of TRPV1 antagonist into the facial skin of M1TP CFA-treated rats, the decrease of HWTs in response to mechanical and heat stimulation of the facial skin was significantly reversed. The present findings suggest that the excitability of TG neurons is enhanced upon tooth pulp inflammation, leading to overexpression of TRPV1 in TG neurons innervating the facial skin, and that satellite glial cells are also activated, resulting in the development of ectopic orofacial pain.
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*中澤 遼太郎, 清水 康平, 田中 有弥, 石井 久夫
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*石井 久夫, 木全 俊輔, 松﨑 厚志, 井手 一郎, 丸山 泰一, 山口 雄生, 清水 康平, 田中 有弥
会議録・要旨集
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Joji Fujisaki, Takahiro Tanaka, Jyundai Kato, Tetsuya Saito, Kohei Yano, Yutaka Shimizu, Toshikatsu Sada, Kouichi Kitazume, Akira Fujita, Yuji Kira
ジャーナル
フリー
An unusual case of primary cardiac lymphoma presenting as restrictive cardiomyopathy with arrhythmia is reported in a 72-year-old woman who was admitted for evaluation of exertional dyspnea and palpitations. Electrocardiography (ECG) showed atrioventricular dissociation and right heart cardiac catheterization revealed a typical `dip-and-plateau' waveform. Restrictive cardiomyopathy was suspected because computed tomography (CT) did not reveal pericardial thickening, calcifications, or an effusion. Heart failure initially improved with diuretic therapy, but subsequently worsened, and the patient experienced a syncopal episode. ECG showed atrial fibrillation, and CT revealed a large mass in the right atrium and multiple tumors in the liver, which needle biopsy confirmed as diffuse large B-cell lymphoma. Chemotherapy induced complete remission, and her heart failure markedly improved. The `dip-and-plateau' waveform was no longer detected on repeat cardiac catheterization and the ECG showed restoration of sinus rhythm. Clinically, the diagnosis was primary cardiac lymphoma. (
Circ J 2005;
69: 249 - 252)
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