ACTA HISTOCHEMICA ET CYTOCHEMICA
Online ISSN : 1347-5800
Print ISSN : 0044-5991
ISSN-L : 0044-5991
HISTOCHEMICAL DEMONSTRATION OF CHOLINESTERASE ACTIVITY IN TISSUES OF THE CARP AND EFFECT OF DDVP ON ITS ACTIVITY IN SITU
SHINICHI YAMANEARIFUMI KINOSEN-ICHI TESHIMA
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1974 Volume 7 Issue 2 Pages 167-174

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Abstract
Cholinesterase activity of the optic lobe, cerebellum, medulla oblongata, retina, intestine, liver, kidney, heart and body muscles were examined histochemically using the carp.
Intense cholinesterase activity was demonstrated in the stratum griseum periventriculare, granular layer of the cerebellum, inner plexiform layer of the retina, lamina propria and submucosa of the intestine, septum of sublayers of the muscularis of the intestine, Malpighian corpuscle of the kidney, wall of ventricle of the heart, and sarcolemma of body muscles. In many other tissue layers studied moderate or weak activities of the enzyme were demonstrated.
Effect of 0, 0-dimethyl-2, 2-dichloro-vinyl phosphate (DDVP) on the cholinesterase activity in situ was performed after sublethal exposure of the fish to DDVP (25ppm for about 45min). DDVP exposure abolished cholinesterase activity in many of the tissues examined including the stratum griseum periventriculare, sarcolemma, and liver. This exposure abated the enzyme activity in all other tissues studied.
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© the Japan Society of Histochemistry and Cytochemistry
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