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Volume 7 (1974)
- Issue 4 Pages 259-
- Issue 3 Pages 181-
- Issue 2 Pages 105-
- Issue 1 Pages 1-
Volume 7, Issue 2
Displaying 1-8 of 8 articles from this issue
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SETSUYA FUJITA, TSUKASA ASHIHARA, MASARU FUKUDA, OSAMU TAKEOKA1974 Volume 7 Issue 2 Pages 105-111
Published: 1974
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSCombination of DNA cytofluorometry and tritiated thymidine autoradiography enables us to measure absolute length of S-phase in a cell population, with a single sampling. Different ploidy classes in a mixed population can be measured separately according to difference in DNA contents. Proliferating cells in the regenerating rat liver are studied by this method and the S-phase is estimated at 6.7hr and 8hr for diploid and tetraploid classes, respectively. After cumulative labeling from 20 to 35hr after hepatectomy, it was found that most labeled hepatocytes do not return to the diploid level, thereby indicating that the regenerative proliferation of the hepatocytes is not a reversible process. In the initial stage up to 30hr after hepatectomy, both diploid and tetraploid cells synthesize DNA. From 30hr to 35hr after hepatectomy, DNA-synthetic cells are mostly of tetraploid class. At 48 post-operative hours, again both diploid and tetraploid cells are synthesizing DNA. Throughout the regenerative period, the tetraploid cells synthesize DNA, divide and return to the tetraploid G1 cells. They seem to play the most important role in the regeneration of the adult rat liver.View full abstractDownload PDF (644K) -
KUNIYASU SHIMOKAWA1974 Volume 7 Issue 2 Pages 112-125
Published: 1974
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSLight microscopic and histochemical investigations were performed on a total of 329 CBA×C57BL/6 F1 hybrid mice which were subcutaneously transplanted with Gardner osteosarcoma 519 between the 80th to the 95th transplant generations. The transplantability was 92.4%, and the survival time was 63±23 days. Metastases were seen only in the lung in 45 (21.0%) of the 214 cases which survived over 40 days. The grafted tumors were palpable at about 10 days and became the size of the head of the little finger at about 50 days after inoculation. When the sizes of the tumors were small they mainly consisted of spindle cells with many mitotic figures, and strong activities of both alkaline and acid phosphatase and PAS reaction-positive glanules were present. Multinucleated giant cells, reticular stroma and osteoid or bone tissues appeared with tumor growth, but the numbers of mitotic figures and the activity of alkaline phosphatase were reduced. Thus, the author classified this sarcoma in four histological types; the spindle cell type, the giant cell type, the reticular stroma type and the osteoid-bone type. The spindle cell type was considered as a proliferating phase and the reticular stroma and osteoid-bone types were as a differentiating phase. The functions of this tumor to form bone or osteoid tissue and to increase the levels of the serum alkaline phosphatase of the host were proved to be retained after 95 transplant generations; therefore, this sarcoma was regarded as a kind of functioning tumor with the above functions.View full abstractDownload PDF (11830K) -
Y. IBATA, T. MATSUURA, Y. NOJYO, T. INOUE, Y. SANO1974 Volume 7 Issue 2 Pages 126-139
Published: 1974
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSThe influence of 5-hydroxydopamine (5-OHDA) and 6-hydroxydopamine (6-OHDA) upon the aminergic neurons of the central nervous system was investigated by fluorescence histochemistry and electron microscopy using the neostriatum, the hypothalamus, including the median eminence, and the substantia nigra of albino rats and cats. After the administration of 5-OHDA into the lateral ventricle or third ventricle, aminergic terminals containing small dense-cored vesicles (400-600Å in diameter) and large cored vesicles (700-1, 000Å in diameter) appeared in the structures listed above. A number of dense-cored vesicles (600-800Å in diameter) were found in the perikarya of some nerve cells of the substantia nigra after treatment with 5-OHDA. It was established that 5-OHDA can be utilized as a marker of both central noradrenaline and dopamine neurons and that endogenous amines are stored in the small vesicles as well as in the large densecored veiscles. On the other hand, intensive depletion of both noradrenaline and dopamine fluorescence occurred in all regions except the external layer of the median eminence after treatment with 6-OHDA. The nerve cell bodies of the substantia nigra, containing dopamine in their perikarya, were less affected by 6-OHDA than the terminals. The administration of 6-OHDA in excess of 500μg produced degeneration of aminergic terminals in animals surviving more than 2 days. However, for short survival times of 1hr, we observed terminals containing small dense cored vesicles similar to those seen after administration of 5-OHDA. A marked depletion of amine fluorescence without ultrastructural damage was also observed in the aminergic terminals.View full abstractDownload PDF (18719K) -
N. J. CHINOY, S. M. LALIWALA, P. Y. PARMAR, V. C. SHAH1974 Volume 7 Issue 2 Pages 140-146
Published: 1974
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSA histochemical investigation on the content of ascorbic acid in chick embryos of 30 hours to 12 day age group was undertaken using an improved histochemical technique. High deposition of ascorbic acid in tissues like the developing brain, optic vesicle, gut, liver, kidney and feather germ suggests that actively growing embryonic tissues are rich in this vitamin and possess its faster turnover. The localization of ascorbic acid in the mesenchymal areas may be related to the synthesis of collagen. Our findings support the view of some others that ascorbic acid and its free radical are important sources of electron energy for biosynthetic processes in growing tissues.View full abstractDownload PDF (8056K) -
IKUKO NAGATSU1974 Volume 7 Issue 2 Pages 147-157
Published: 1974
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSAn improved immunocytochemical method was developed in order to reduce non-specific staining, and it was compared with an improved immunofluorescent method. In these improved methods, antiserum to rabbit immunoglobulin (IgG) was purified by affinity chromatography, and then conjugated with peroxidase by the Nakane's method for the purpose of immunocytochemical method and, also with FITC for immunofluorescent method. By these procedures, non-specific staining became very low comparing with the method using commercially available fluorescein isothiocyanate (FITC)-conjugate, and specificity in the staining was increased.
Another new trial was made for the immunofluorescent method. The BG12 exciting filter had been frequently used for fluorescence microscopy, but the use of a Leitz KP490 filter and a BG38 filter instead of the BG12 was found to be effective to reduce non-specific fluorescence and to increase the fluorescence of specific staining.View full abstractDownload PDF (11365K) -
TERUO IWAMASA, HIDETOSHI KOJIMA, SHOTARO HISAMITSU, SHINICHI TOKUMITSU ...1974 Volume 7 Issue 2 Pages 158-166
Published: 1974
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSUltrastructural appearances and physico-chemical properties of fetal rat liver glycogen were studied. Under electron microscopy, fetal liver glycogen particles were observed as small spheroidal branching bodies which were slightly larger than adult muscle glycogen particles showing 500-800Å in diameter. However, a few of them were demonstrated as large branching structures and were similarly large in size to adult liver glycogen particles. The average chain length and the degree of branching of fetal glycogen determined by the periodate oxidation method resembled adult liver glycogen. The ORD and CD absorption spectra waves of fetal glycogen, measured while adding iodine, showed positive Cotton effect at 540nm which was different from that of adult liver glycogen.
Ultracentrifugation analysis demonstrated the sedimentation coefficient of 106.9s. Among adult muscle and hepatoma glycogen, hepatoma glycogen was most similar to fetal glycogen in reference to the physico-chemical examinations as previously described (2, 4, 5).View full abstractDownload PDF (7904K) -
SHINICHI YAMANE, ARIFUMI KINO, SEN-ICHI TESHIMA1974 Volume 7 Issue 2 Pages 167-174
Published: 1974
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSCholinesterase activity of the optic lobe, cerebellum, medulla oblongata, retina, intestine, liver, kidney, heart and body muscles were examined histochemically using the carp.
Intense cholinesterase activity was demonstrated in the stratum griseum periventriculare, granular layer of the cerebellum, inner plexiform layer of the retina, lamina propria and submucosa of the intestine, septum of sublayers of the muscularis of the intestine, Malpighian corpuscle of the kidney, wall of ventricle of the heart, and sarcolemma of body muscles. In many other tissue layers studied moderate or weak activities of the enzyme were demonstrated.
Effect of 0, 0-dimethyl-2, 2-dichloro-vinyl phosphate (DDVP) on the cholinesterase activity in situ was performed after sublethal exposure of the fish to DDVP (25ppm for about 45min). DDVP exposure abolished cholinesterase activity in many of the tissues examined including the stratum griseum periventriculare, sarcolemma, and liver. This exposure abated the enzyme activity in all other tissues studied.View full abstractDownload PDF (5848K) -
S. V. S. RANA1974 Volume 7 Issue 2 Pages 175-178
Published: 1974
Released on J-STAGE: October 28, 2009
JOURNAL FREE ACCESSDownload PDF (3862K)
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