Non-dissociative Small Fragments of Glycinin-T

Abstract

The small fragments (P) were detected in glycinin-T (tryptic digestion intermediate of glycinin) by acetic acid-urea gel electrophoresis. Urea or 0.5M acetic acid was needed for dissociation of them from glycinin-T. In the presence of urea, P fragments were separated from ISTs by use of solubility difference and Sephadex G-50 chromatography. Two bands of P fragments were observed on acetic acid-urea gels whereas a single band was on SDS-urea gels suggesting that the molecular weights of the two are almost the same. The fragments were also separable on a column of DEAE Sepharose CL-6B. They were isolated and the molecular weight of them was 9600.