Biological and Pharmaceutical Bulletin
Online ISSN : 1347-5215
Print ISSN : 0918-6158
ISSN-L : 0918-6158
Regular Articles
cDNA Cloning of the Sry-Related Gene Sox6 from Rat with Tissue-Specific Expression
Masanori NaraharaAkihisa YamadaMichiko Hamada-KanazawaYuichi KawaiMasaharu Miyake
Author information
JOURNAL FREE ACCESS

2002 Volume 25 Issue 6 Pages 705-709

Details
Abstract
A cDNA encoding rat homologue of the previously characterized mouse Sox6 was isolated by a polymerase chain reaction (PCR) cloning strategy. Comparison of this cDNA with homologous mouse, human and rainbow trout cDNA exhibited an overall amino acid sequence identity of 99.6, 89.3 and 76.3% respectively. The leucine-zipper and HMG-box motif were almost completely conserved between these homologues. The expression of Sox6 was determined in rat by Northern hybridization and Real-time quantitative reverse transcription (RT)-PCR. rSox6 (rat Sox6) was specifically expressed in the neonatal brain and adult testis with Northern blotting. Real-time quantitative RT-PCR for the determination of Sox6 mRNA was examined. The rSox6 was expressed in the neonatal brain and adult testis as well as by Northern blotting and also expressed in the adult eyeball and slightly in the ovary.
Content from these authors
© 2002 The Pharmaceutical Society of Japan
Previous article Next article
feedback
Top