STRUCTURAL ANALYSIS OF A LOW-SULFATED CHONDROITIN SULFATE CHAIN IN HUMAN URINARY TRYPSIN INHIBITOR

Hidenao TOYODA; Shojiro KOBAYASHI; Shinobu SAKAMOTO; Toshihiko TOIDA; Toshio IMANARI

doi:10.1248/bpb.16.945

Hidenao TOYODA, Shojiro KOBAYASHI, Shinobu SAKAMOTO, Toshihiko TOIDA, Toshio IMANARI

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Keywords: trypsin inhibitor, chondroitin sulfate, glycosaminoglycan, proteoglycan, urine

JOURNAL FREE ACCESS

1993 Volume 16 Issue 9 Pages 945-947

DOI https://doi.org/10.1248/bpb.16.945

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Abstract

The low-sulfated chondroitin 4-sulfate(LSC) chain from human urinary trypsin inhibitor was purified and the structure was characterized. After hyaluronidase SD digestion of LSC, an oligosaccharide which contains the linkage region could be botained. The structure of oligosaccharide was analyzed by HPLC and 500 MHz ¹H-NMR spectroscopy. The analytical results revealed that 4-O-sulfo GalNAc residues were located in the neighborhood of the linkage region.

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