High Level Expression of Recombinant Chicken Interferon-α Using Baculovirus

Abstract

Bioactive recombinant chicken interferon-α (ChIFN-α) was expressed in a baculovirus system. For easy purification, it was expressed as ChIFN-α bearing histidine hexamer (His-tag) at C-terminal, designated ChIFN-αHis. The expressed proteins were detected by SDS-PAGE analysis with Coomassie brilliant blue staining as around 23 and 19 kDa bands thought to be immature and matured ChIFN-αHis respectively. The purified ChIFN-αHis with a nickel chelated column showed anti-viral activity in vitro.