UTP causes interleukin (IL)-6 production via mRNA expression through P2Y
2/P2Y
4 receptors in human HaCaT keratinocytes. In the present study, we analyzed the mechanism of UTP-induced IL-6 production in these cells. UTP, an agonist of P2Y
2/P2Y
4 receptors, induced phosphorylation of extracellular signal-regulated kinase (ERK) in a concentration- and time-dependent manner. PD98059, a MEK (mitogen-activated protein kinase kinase) inhibitor, and BAPTA-AM [
O,O'-bis(2-aminophenyl)ethyleneglycol-
N,N,N',N'-tetraacetic acid, tetraacetoxymethyl ester], an intracellular Ca
2+ chelator, reduced UTP-induced ERK phosphorylation and IL-6 mRNA expression. 2-APB [(2-aminoethoxy)diphenylborane], an inositol 1,4,5-trisphosphate (IP
3)-receptor antagonist, inhibited UTP-induced IL-6 mRNA expression; and the action of A23187, a Ca
2+ ionophore, resembled the action of UTP. In contrast, protein kinase C (PKC) downregulation and pertussis toxin did not affect UTP-induced IL-6 mRNA expression, suggesting that PKC and G
i are not involved in the UTP-induced IL-6 production. However, AG1478, an epidermal growth factor (EGF)-receptor inhibitor, partially decreased UTP-induced ERK phosphorylation and IL-6 expression. These results suggest that UTP-induced IL-6 production is in part mediated via phosphorylation of ERK through G
q/11/IP
3/[Ca
2+]
i and transactivation of the EGF receptor.
View full abstract