Abstracts of the Annual Meeting, The Japanese Society of Sericultural Science

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Enzyme activity and gene expression of GTP-CH__I__during color pattern development in wings of the cabbage butterfly , Pieris rapae crucivora

Keiko Fukushima, Hiroshi Sawada, Hajime Fugo, Akihiro Yoshida, Motoko ...

Session ID: 101
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.1.0

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Accumulation of uric acid concernd with larval color dimorphism in the sweet potato hornworm, Agrius convolvuli.

Ayano Otake, Motoko Nakagoshi, Kunikatsu Hamano

Session ID: 102
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.2.0

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Molecular mechanisms of melanization in the larval body marking of a swallowtail butterfly, Papilio xuthus.

Ryo Futahashi, Haruhiko Fujiwara

Session ID: 103
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.3.0

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Cross-linking of GGYGG peptide found in hard proteins by phenol oxidase

Haruhiko Fujiwara

Session ID: 104
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.4.0

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Effect of anoxia on promoting diapause development

Kurako Kidokoro, Yoshikazu Ando

Session ID: 105
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.5.0

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The role of a serotonin signal transduction pathway for diapause termination in Antheraea pernyi

Susumu Hiragaki, Taketo Tsugehara, Yoshihiro Fuziwara, Makio Takeda

Session ID: 106
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.6.0

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TIME-EA4 time-measurement under the control by PIN at 25˚C

Xiaonan Ti, Naoki Tani, Hidenori Kai, Minoru Isobe

Session ID: 107
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.7.0

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Diapause duration-timer in Bombyx eggs with special reference to the PIN action on EA4

Naoki Tani, Nobuhiko Tuzuki, Etuko Morigami, Xiaonan Ti, Hidenori Kai, ...

Session ID: 108
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.8.0

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Analysis of the species-specificity determination mechanism of the prothoracicotropic hormone activity using the baculovirus vector

Takamasa Ishizuka, Jun Kobayashi, tetsuro yoshimura, yoshiaki Tanaka

Session ID: 109
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.9.0

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Occurrence of melanosis in the hemocoel of the silkworm larva and colorization of the athrocytes after injection of inactivated E. coli

Masao Nagata, Chie Owa, Fugaku Aoki

Session ID: 110
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.10.0

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The silkworm larvae were apparently healthy after injection of heat-inactivated Bacillus thuringiensis. The heat-inactivated E. coli, however, induced melanosis in the hemocoel and colorization of athrocytes(nephrocytes) such as pericardial cells, peritracheal glandular cells and subesophageal body. These results suggested that prophenoloxidase cascade in the hemolymph was activated by E. coli and produced melanin was incorporated into the athrocytes.

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Peritracheal athrocytes(nephrocytes) in the silkworm, Bombyx mori, and their existence among several lepidopteran insects

Chie Owa, Fugaku Aoki, Masao Nagata

Session ID: 111
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.11.0

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Distribution of carotenoid binding protein in the midgut and silk gland

Kozo Tsuchida, Jeniffer Gardetto, Yutaka Kobayashi, Hiroko Tabunoki, M ...

Session ID: 112
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.12.0

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We utilized the YI, Y+^I, +^YI and +^Y+^I strains to detect cellular distribution of CBP.CBP has been isolated from the silk gland of Bombyx mori. Western blotting analysis demonstrated the presence of CBP only in the anteior part of midgut and the middle of the silk gland. Immunocytochemistry confirmed the presence of CBP in strains with the dominant Y gene and also verified the localization of CBP in the brush border of the midgut epithelial cells

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Trial to quantify of diapause hormone in the pupal hemolymph and the mature egg of the silkworm, Bombyx mori

Norio KITAGAWA, Kunio IMAI, Kunihiro SHIOMI, Teruyuki NIIMI, Okitsugu ...

Session ID: 113
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.13.0

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Firing activity patterns of neurosecretory cells producing diapause hormone and its family peptides in female silkmothsBombyx mori

Toshio Ichikawa

Session ID: 114
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.14.0

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Firing activity of "diapause-hormone" producing cells and hormonal activity in male silkmoths, Bombyx mori

Akiko Suenobu, Toshio Ichikawa

Session ID: 115
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.15.0

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Isolation of repressive factor-binding protein in diapausing pharate first instar larvae of Antheraea yamamai

Shiduko Abe, Ping Yang, Koichi Suzuki

Session ID: 116
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.16.0

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Identification of CNS-associated protein used the antiserum of KK-42-binding protein from diapausing pharate first instar lavae of the wild silkmoth Antheraea yamamai

naoyuki saito, nobuko sakamoto, takayuki shimizu, koichi suzuki

Session ID: 117
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.17.0

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Structure and gene analysis of a repressive factor in the wild silkmoth Antheraea yamamai

ping yang, ying an, hiromasa tanaka, yoshiko sawa, sizuko abe, koichi ...

Session ID: 118
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.18.0

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Region-specific expression of ecdysone receptor (BmEcR) isoforms during pupal wing development in the silkworm, Bombyx mori.

Eigo Suyama, Tomoko Matsunaga, Kunihiro Shiomi, Manabu Kamimura, Haruh ...

Session ID: 120
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.19.0

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Ecdysone-activated signalling cascade involved in the regeneration of midgut cells in Bombyx mori.

Yoshiaki Tanaka, Fumiko Yukuhiro

Session ID: 121
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.20.0

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Hormonal control of metamorphic development of the silkgland in the silkworm, Bombyx mori.

SUMIHARU NAGAOKA, MAYUKO OKUMURA

Session ID: 122
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.21.0

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pH estimation in situ in the glandular lumen of the silk gland

Masaaki Azuma

Session ID: 123
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.22.0

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pH of the liquid silk in the glandular lumen was estimated in situ by injecting the pH-sensitive dye, phenol red. In the 5th instar feeding larvae of Bombyx mori, the pH value of the lumen in the middle part of the middle silk gland (MSG-mid) was around 5.5. That of the posterior part of MSG (MSG-post) was around pH 6.5. That in the posterior silk gland (PSG) was pH 7-8. In the spinning larvae, pH at the MSG-mid was neutralized to >pH 6. These tendency was also confirmed in the silk gland of Samia cynthia ricini.

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Functional analysis of PKR inhibitor in cultured silkworm cells

Kosuke Sakashita, Takahiro Kusakabe, Chisa Aoki, Yutaka Kawaguchi, Kat ...

Session ID: 124
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.23.0

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Hematopoietic lineage of the silkworm

Yuichi Nakahara, Yasushi Kanamori, Makoto Kiuchi, Manabu Kamimura

Session ID: 125
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.24.0

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Polydnavirus and venom of parasitoid wasp regulate the host hemocytes

Tokiyasu Teramoto, Toshiharu Tanaka

Session ID: 126
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.25.0

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Regeneration of hemopoietic organs irradiated with heavy ion beams in the silkworm, Bombyx mori : Phagocytosis of irradiated cells by hemocytes in circulation

Erjun Ling, Kana Fukamoto, Koji Shirai, Rensuke Kanekatsu, Kenji Kiguc ...

Session ID: 127
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.26.0

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Purification and characterization of the granular phenol oxidase from cuticle of the silkworm, Bombyx mori mori

Koji Shirai, Masaaki Ashida, Masanori Ochiai, Rensuke Kanekatsu, Kenji ...

Session ID: 128
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.27.0

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The mode of cell prolifelation during the cyst formation of the inplanted larval integument and wound healing in the sweet potato hornworm , Agrius convolvuli

Kana Fukamoto, Koji Shirai, Rensuke Kanekatsu, Kenji Kiguchi

Session ID: 129
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.28.0

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BH4 biosynthetic pathway; Relationship of silkworm CR and human AKR family members

Mayuko Tabata, Teruhiko Iino, Shin-ichiro Takikawa, Hiroshi Sawada, Ke ...

Session ID: 130
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.29.0

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Effect of validoxylamineA on Trehalase from anterior silk gland of silkworm,Bombyx mori

keiko sadakane, shuya shimada, shunitsu kou, toshiharu furusawa, yoshi ...

Session ID: 131
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.30.0

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In vitro culture of embryos in the silkworm

Ken-ichi Iwata, Yoshihiro Fujiwara, Makio Takeda

Session ID: 132
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.31.0

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Analysis of homologous recombination in cultured silkworm cells

HIROAKI MON, TAKAHIRO KUSAKABE , CHISA AOKI, JAE MAN LEE, YUTAKA ...

Session ID: 133
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.32.0

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Bombyxin stimulates midgut stem cell proliferation of lepidopteran larvae in vitro

Shintaro Goto, Marcia Loeb, Makio Takeda

Session ID: 134
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.33.0

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Temporal Profiles of Fat Body Protein in the Developing Larvae of Silkworm, Bombyx mori

Pingbo Zhang, Kohji Yamamoto, Yutaka Bannno, Hiroshi Fujii

Session ID: 135
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.34.0

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Many proteins including 30 kDa protein, storage protein, chymotrypsin inhibitor and so on had been shown that they were synthesized in the larva stages of fat body of silkworm, secreted into hemolymph, and then transferred between different organs and cells with the physiological development. Protein profiles of fat body in the developing silkworm larvae were analyzed by means of two-dimensional gel electrophoresis (2-DE) and mass spectrometry (MS). The analysis of fat body gave rise to a protein map comprising approximately 1000 spots detectable by silver staining following high 2-DE with a pH range of 3-10 and a mass range 12-100 kDa. To obtain landmarks for fat body, 142 spots were identified by peptide mass fingerprinting using MALDI-TOF-MS. Spot matching, alignment and quantitation was performed with PDQuest software. Comparison of temporal profiles of fat body protein in the developing silkworm larvae revealed significant changes in the expression of proteins during development.

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cDNA cloning of a novel trehalase from the silkworm, Bombyx mori

Kanako Mitsumasu, Teruyuki Niimi, Okitsugu Yamashita, Toshinobu Yaginu ...

Session ID: 136
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.35.0

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Use of bicistronic vector to obtain overexpression cell line

Takuji Maeda, Takahiro Kusakabe, Nobuhiko Nakashima, Norihiro Shibuya, ...

Session ID: 137
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.36.0

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Structure and function of Bombyrin in the central nervous system of Bombyx mori

masato sakai, koichi suzuki

Session ID: 138
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.37.0

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Transgenic expression of diapausin, a diapause-specific peptide from Gastrophysa atrocyanea, in Bombyx mori using piggybac vector

Hiromasa Tanaka, Morikazu Imamura, Toshio Kanda, Toshiki Tamura, Koich ...

Session ID: 201
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.38.0

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Establishment of transgenic silkworm to produce complex N-linked glycans

Masafumi Yamamoto, Masafumi Yamao, Hajime Mori, Donald L. Jarvis

Session ID: 202
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.39.0

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NMR study on structure of transcriptional regulatory protein of fibroin gene FMBP1

Makoto Demura, Kyosuke Kawaguchi, Tomoyasu Aizawa, Katsutoshi Nitta, S ...

Session ID: 203
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.40.0

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Effect of insert size on efficiency in construction of transgenic silkworm using piggyBac

Toshiki TAMURA, Toshio KANDA, Nobuo KUWABARA, Guo-Xing QUAN, Morikazu ...

Session ID: 204
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.41.0

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The ability of complex N-glycan addition in Anteraea pernyi insect cell line (AnPe) demonstrated of by 2D-sugar mapping technique.

Masahiro Nagaya, Jun Kobayashi, Noriko Takahashi, Koichi Kato, Tetsuro ...

Session ID: 205
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.42.0

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Cytotoxic activity in Zaptychopsis buschi extracts against rat hepatoma cells

Shiro Go-ukon, Koichi Suzuki, Norio Doi

Session ID: 206
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.43.0

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Providing ceramics as silkworms feed to generate organization consisting substances of ceramics

Fukuju Miyazawa

Session ID: 207
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.44.0

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Tests were conducted to observe as to whether the cocoon thread would consist substances of ceramics when silkworms were provided seramics food. As a result ceramics incorporated cocoon thread was effectively generated when 25%or less of the dried food composed of ceramics,Also,in the feeding time,the second half of 5 inster was effective especially. Furthermore,the qualitative analysis regarding the organization of the cocoons exhibited that the blood silk thread gland pupa,and casting off shell also contained substances of ceramics,These are the result which overrules common sense so far.

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All the year round germfree sericulture with three times feeding method in Osaka city

Fujiyoshi Matsubara, Keiko Gouda, Ruiying Chen , Daizaburou Yasunaga, ...

Session ID: 208
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.45.0

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Non-steamed artificial diet for thesilkworm, Bombyx mori

Masatoshi Ichida, Noriyuki Harada, Yuko Norimoto, Atsuko Ichida, Keiko ...

Session ID: 209
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.46.0

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Development of continuous silkworm larva freezing system.

MASANOBU OHURA

Session ID: 210
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.47.0

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Improvement of silkworm hemolymph collecting system-attached to a feeder of silkworm

Toru Kobayashi, Tamotu Takahashi, Yasushi Tamada

Session ID: 211
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.48.0

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Involvement of Rel protein in the transcriptional regulation of A. d. coleoptericin A gene

Aki Sagisaka, Hiromitsu Tanaka, Seiichi Furukawa, Minoru Yamakawa

Session ID: 212
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.49.0

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Identification of transcriptional activation domain of BmRel A and BmRel B

Hiromitsu TANAKA, Seiichi FURUKAWA, Hiroshi NAKAZAWA, Aki SAGISAKA, Mi ...

Session ID: 213
Published: 2003
Released on J-STAGE: May 27, 2004

DOIhttps://doi.org/10.11416/jsss.2003.0.50.0

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