Hydroxyapatite Maturity in the Calcified Cartilage and Underlying Subchondral Bone of Guinea Pigs with Spontaneous Osteoarthritis: Analysis by Fourier Transform Infrared Microspectroscopy

Mitsuhiko Sato; Makoto Wada; Norio Miyoshi; Yoshiaki Imamura; Sakon Noriki; Kenzo Uchida; Shigeru Kobayashi; Takafumi Yayama; Kohei Negoro; Michiyo Fujimoto; Masaru Fukuda; Hisatoshi Baba

doi:10.1267/ahc.37.101

REGULAR ARTICLE

Hydroxyapatite Maturity in the Calcified Cartilage and Underlying Subchondral Bone of Guinea Pigs with Spontaneous Osteoarthritis: Analysis by Fourier Transform Infrared Microspectroscopy

Mitsuhiko Sato, Makoto Wada, Norio Miyoshi, Yoshiaki Imamura, Sakon Noriki, Kenzo Uchida, Shigeru Kobayashi, Takafumi Yayama, Kohei Negoro, Michiyo Fujimoto, Masaru Fukuda, Hisatoshi Baba

Author information

Mitsuhiko Sato
Department of Orthopaedics and Rehabilitation Medicine, School of Medicine, University of Fukui
Department of Pathology, School of Medicine, University of Fukui
Makoto Wada
Department of Orthopaedics and Rehabilitation Medicine, School of Medicine, University of Fukui
Norio Miyoshi
Department of Pathology, School of Medicine, University of Fukui
Yoshiaki Imamura
Department of Pathology, School of Medicine, University of Fukui
Sakon Noriki
Department of Pathology, School of Medicine, University of Fukui
Kenzo Uchida
Department of Orthopaedics and Rehabilitation Medicine, School of Medicine, University of Fukui
Shigeru Kobayashi
Department of Orthopaedics and Rehabilitation Medicine, School of Medicine, University of Fukui
Takafumi Yayama
Department of Orthopaedics and Rehabilitation Medicine, School of Medicine, University of Fukui
Kohei Negoro
Department of Orthopaedics and Rehabilitation Medicine, School of Medicine, University of Fukui
Michiyo Fujimoto
Department of Orthopaedics and Rehabilitation Medicine, School of Medicine, University of Fukui
Masaru Fukuda
Department of Pathology, School of Medicine, University of Fukui
Hisatoshi Baba
Department of Orthopaedics and Rehabilitation Medicine, School of Medicine, University of Fukui

Keywords: osteoarthritis, calcified cartilage, hydroxyapatite, crystallinity, Fourier transform infrared microspectroscopy

JOURNAL FREE ACCESS

2004 Volume 37 Issue 2 Pages 101-107

DOI https://doi.org/10.1267/ahc.37.101

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Abstract

The aim of the present study was to investigate the quantity and quality of hydroxyapatite (HA) in the zone of calcified cartilage of articular cartilage and underlying subchondral bone by Fourier transform infrared microscopy (FTIRM). We hypothesized that the amount of HA deposited in osteoarthritis (OA) might differ from normal cartilage, and that such differences might be significant in the pathology of OA. The tibial epiphysis of the male Hartley strain for the spontaneous OA model and the control Strain 13 guinea pigs (12-month old) were examined. The mineral:matrix ratio was determined by integrating areas of υ1, υ3 bands at 900-1200 cm^-1 to Amid 1 band at 1585-1725 cm ^-1. The CO₃:PO₄ ratio was determined by integrating areas of υ2 carbonate band at 850-900 cm^-1 to υ1, υ3 bands. In Hartley strain, the mean mineral:matrix ratio of the medial side was significantly lower than the lateral side at the zone of the calcified cartilage and subchondral bone. The mean CO₃:PO ₄ ratio was significantly higher in the medial side than the lateral one at the zone of the calcified cartilage and subchondral bone. In contrast, the results obtained from Strain 13 showed no significant differences between the two sides. Our results showed diminished deposition and increased maturation of HA in Hartley strain. These findings may correlate with the pathological process of knee OA, and indicate that FTIRM is a useful tool for determining the variability of HA in OA.

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