Synthesis of (R)-1,3-Butanediol by Enantioselective Oxidation Using Whole Recombinant Escherichia coli Cells Expressing (S)-Specific Secondary Alcohol Dehydrogenase

Hiroaki YAMAMOTO; Akinobu MATSUYAMA; Yoshinori KOBAYASHI

doi:10.1271/bbb.66.925

Microbiology & Fermentation Technology Notes

Synthesis of (R)-1,3-Butanediol by Enantioselective Oxidation Using Whole Recombinant Escherichia coli Cells Expressing (S)-Specific Secondary Alcohol Dehydrogenase

Hiroaki YAMAMOTO, Akinobu MATSUYAMA, Yoshinori KOBAYASHI

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Keywords: secondary alcohol dehydrogenase, (R)-1,3-butanediol, NAD⁺-regeneration, enantioselective oxidation

JOURNAL FREE ACCESS

2002 Volume 66 Issue 4 Pages 925-927

DOI https://doi.org/10.1271/bbb.66.925

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Abstract

The synthesis of (R)-1,3-butanediol (BDO) from its racemate was studied using whole cells of recombinant Escherichia coli expressing an (S)-specific secondary alcohol dehydrogenase (CpSADH) from Candida parapsilosis by enantioselective oxidation. Under the optimized conditions, the yield of (R)-1,3-BDO reached 72.6 g/l, with a molar recovery yield of 48.4% from a racemate of 15% and an optical purity of 95% ee.

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