Common Amino Acid Sequences Deduced from Coding Exons of the Porcine FGF4 Gene in Two Breeds and Production of the Encoded Protein in Escherichia coli

Saiko SUGAWARA; Toshihiko ITO; Hitoshi SUZUKI; Toshikiyo TAKAHASHI; Jun-ichi KONISHI; Masaki KOBAYASHI; Sho SATO; Yuki MORI; Kano KASUGA; Ikuo KOJIMA; Tomokazu FUKUDA; Masayuki KOBAYASHI

doi:10.1271/bbb.120698

Biochemistry & Molecular Biology Regular Papers

Common Amino Acid Sequences Deduced from Coding Exons of the Porcine FGF4 Gene in Two Breeds and Production of the Encoded Protein in Escherichia coli

Saiko SUGAWARA, Toshihiko ITO, Hitoshi SUZUKI, Toshikiyo TAKAHASHI, Jun-ichi KONISHI, Masaki KOBAYASHI, Sho SATO, Yuki MORI, Kano KASUGA, Ikuo KOJIMA, Tomokazu FUKUDA, Masayuki KOBAYASHI

Author information

Saiko SUGAWARA
Graduate School of Bioresource Sciences, Akita Prefectural University
Toshihiko ITO
Graduate School of Bioresource Sciences, Akita Prefectural University
Hitoshi SUZUKI
Akita Prefectural Livestock Experiment Station
Toshikiyo TAKAHASHI
Akita Prefectural Livestock Experiment Station
Jun-ichi KONISHI
Akita Prefectural Livestock Experiment Station
Masaki KOBAYASHI
Akita Hokubu Livestock Hygiene Service Center
Sho SATO
Graduate School of Bioresource Sciences, Akita Prefectural University
Yuki MORI
Graduate School of Bioresource Sciences, Akita Prefectural University
Kano KASUGA
Graduate School of Bioresource Sciences, Akita Prefectural University
Ikuo KOJIMA
Graduate School of Bioresource Sciences, Akita Prefectural University
Tomokazu FUKUDA
Graduate School of Agricultural Sciences, Tohoku University
Masayuki KOBAYASHI
Graduate School of Bioresource Sciences, Akita Prefectural University

Keywords: Escherichia coli, fibroblast growth factor 4, porcine, recombinant, structural gene

JOURNAL FREE ACCESS

2013 Volume 77 Issue 1 Pages 173-177

DOI https://doi.org/10.1271/bbb.120698

View "Advance Publication" version (January 7, 2013).

Details

Abstract

Fibroblast growth factor 4 (FGF4) is considered a crucial gene in the development of mammalian embryos. Here we identified common amino acid sequences predicted from coding exons of the FGF4 gene in five pigs of two breeds, and HispFGF4, a 6× histidine-tagged porcine FGF4, was produced in Escherichia coli. HispFGF4 was purified efficiently from the supernatant of cell lysate by heparin column chromatography. In a porcine embryonic fibroblast cell line, HispFGF4 showed significant mitogenic activities at concentrations as low as 0.001 nM (p<0.01). To the best of our knowledge, this is the first report describing the complete nucleotide sequence of coding exons for the porcine FGF4 protein in two breeds, together with the production of a recombinant, bioactive porcine FGF4 derivative.

Corresponding author

Register with J-STAGE for free!