Genetic and antigenic analysis of Chlamydia pecorum strains          isolated from calves with diarrhea

Akifumi OHTANI; Masahito KUBO; Hiroshi SHIMODA; Kenji OHYA; Tadashi IRIBE; Daiki OHISHI; Daiji ENDOH; Tsutomu OMATSU; Tetsuya MIZUTANI; Hideto FUKUSHI; Ken MAEDA

doi:10.1292/jvms.14-0585

Bacteriology

Genetic and antigenic analysis of Chlamydia pecorum strains isolated from calves with diarrhea

Akifumi OHTANI, Masahito KUBO, Hiroshi SHIMODA, Kenji OHYA, Tadashi IRIBE, Daiki OHISHI, Daiji ENDOH, Tsutomu OMATSU, Tetsuya MIZUTANI, Hideto FUKUSHI, Ken MAEDA

Author information

Akifumi OHTANI
Yamaguchi Chubu Livestock Hygiene Service Center, 671-5 Kagawa, Yamaguchi 754–0897, Japan
Masahito KUBO
Laboratory of Veterinary Pathology, Joint Faculty of Veterinary Medicine, Yamaguchi University, 1677–1 Yoshida, Yamaguchi 753–8515, Japan
Hiroshi SHIMODA
Laboratory of Veterinary Microbiology, Joint Faculty of Veterinary Medicine, Yamaguchi University, 1677–1 Yoshida, Yamaguchi 753–8515, Japan
Kenji OHYA
Laboratory of Veterinary Microbiology, Department of Veterinary Medicine, Faculty of Applied Biological Sciences, Gifu University, 1–1 Yanagido, Gifu 501–1193, Japan
Tadashi IRIBE
Yamaguchi Chubu Livestock Hygiene Service Center, 671-5 Kagawa, Yamaguchi 754–0897, Japan
Daiki OHISHI
Yamaguchi Chubu Livestock Hygiene Service Center, 671-5 Kagawa, Yamaguchi 754–0897, Japan
Daiji ENDOH
School of Veterinary Medicine Department of Veterinary Medicine, Rakuno Gakuen University, 583 Bunkyodai Midorimachi, Ebetsu, Hokkaido 069–8501 Japan
Tsutomu OMATSU
Research and Education Center for Prevention of Global Infectious Diseases of Animals, Tokyo University of Agriculture and Technology, 3–8–1 Harumi-cho, Fuchu, Tokyo 183–0057, Japan
Tetsuya MIZUTANI
Research and Education Center for Prevention of Global Infectious Diseases of Animals, Tokyo University of Agriculture and Technology, 3–8–1 Harumi-cho, Fuchu, Tokyo 183–0057, Japan
Hideto FUKUSHI
Laboratory of Veterinary Microbiology, Department of Veterinary Medicine, Faculty of Applied Biological Sciences, Gifu University, 1–1 Yanagido, Gifu 501–1193, Japan
Ken MAEDA
Laboratory of Veterinary Microbiology, Joint Faculty of Veterinary Medicine, Yamaguchi University, 1677–1 Yoshida, Yamaguchi 753–8515, Japan

Keywords: cattle, Chlamydia pecorum, diarrhea

JOURNAL FREE ACCESS

2015 Volume 77 Issue 7 Pages 777-782

DOI https://doi.org/10.1292/jvms.14-0585

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Abstract

Chlamydia pecorum (designated 22–58) was isolated in 2010 in HmLu-1 cells from the jejunum of a calf which died of necrotizing enterocolitis in Yamaguchi Prefecture, Japan. Immunohistochemical staining identified C. pecorum positive reactions in the jejunal villi. C. pecorum, designated 24–100, was isolated from the feces of a calf with diarrhea in another farm in Yamaguchi Prefecture in 2012. A significant increase in neutralizing antibody titers against C. pecorum was confirmed in paired sera. Nucleotide sequence identities of omp1 genes of the 2 isolates were 100%. The isolates were genetically and antigenically more closely related to C. pecorum Bo/Yokohama strain isolated from cattle with enteritis in Japan than to the other prototype strains, Bo/Maeda isolated from cattle with pneumonia and Ov/IPA isolated from sheep with polyarthritis. These results indicate that C. pecorum strains similar to 22–58 and 24–100 might be endemic in Yamaguchi Prefecture and cause enteric disease in cattle.

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