Anisotropic wetting of machined surfaces occurs in many industrial applications, but there has not been much work on it. In this paper, based on the analysis of the influencing factors of anisotropic wetting from the orthogonal directions, experiments investigating anisotropy wetting have been performed on machined surfaces with a wide range of roughness on four kinds of materials. It is found that roughness and chemical properties of materials exert significant effects on anisotropic wetting on machined surface. Generally, it increases with the mean slop of roughness profile Kr, and decreases with intrinsic contact angle. Anisotropic wetting on hydrophilic materials is distinctly stronger than that on hydrophobic materials. These efforts on researching the effects on anisotropy wetting on machined surfaces have shown practical significance in oil recovery, lubrication and sealing.