Development of rapid detection technique for microorganisms using artificial siderophore-modified electrodes

Abstract

Objective:

Siderophores. Artificial siderophores are artificial molecules that mimic the function of natural siderophores which are chelating molecules produced by microorganisms for Fe ions uptake. In this study, we report a technique for rapid and selective detection of microorganisms using electrodes modified with artificial siderophore molecules with different structures.

Methods:

Artificial siderophore molecules of catecholate-type, hydroxamate-type, and hybrid types containing both were designed and synthesized as iron ion capturing sites, and were modified on the electrode surface.

Results:

The adsorption behavior of various microorganisms on the artificial siderophore-modified electrodes with different structures was observed, and it was found that microorganisms were easily adsorbed on the artificial siderophore-modified electrodes which have similar structures to the natural siderophores. We developed the rapid and selective microorganism detection techenique using this phenomenon.