Utilization of Reconstructed Cultured Human Skin Models as an Alternative Skin for Permeation Studies of Chemical Compounds

抄録

Two reconstructed human skin models, Episkin^SM and EpiDerm^TM, have been approved as alternative membranes for skin corrosive/irritation experiments due to their close correlation with animal skin. Such reconstructed human skin models were evaluated as alternative membranes for skin permeation experiments. Seven drugs with different lipophilicities and almost the same molecular weight were used as test penetrants. Relationships were investigated between permeability coefficients (P values) of the seven drugs through six kinds of reconstructed cultured human skin models and human skin. A fairly good relationship in P values was observed between TESTSKIN^TM LSE-high (LSE-high) or EpiDerm^TM and human skin, suggesting that these reconstructed human skin models could be used as alternative membranes for skin permeation experiments. However, the partition parameter, KL, and diffusion parameter, DL^-2 in these reconstructed human skin models were different to those of human skin. Especially, KL values in reconstructed human skin models were very different to those in human skin, even for LSE-high and EpiDerm^TM. Therefore, suitable reconstructed human skin models should be carefully selected on a case by case basis.