Applied Entomology and Zoology
Online ISSN : 1347-605X
Print ISSN : 0003-6862
ISSN-L : 0003-6862
A continuous cell line derived from larval fat bodies of Thysanoplusia intermixta (Lepidoptera: Noctuidae)
Kikuo Iwabuchi
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ジャーナル フリー

2000 年 35 巻 2 号 p. 245-249

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抄録
A continuous cell line has been established from larval fat bodies of a lepidopteran insect, Thysanoplusia intermixta. These cells were cultured in MGM-450 medium. The cell line, designated as TI-1, was a suspension of cells with a doubling time of 36 h and consisted mostly of spindle-shaped cells, although other shapes, notably spherical cells, also existed. The chromosome number of the cell line varied considerably with the mode of 150-179. This cell line was found to be susceptible to Autographa californica NPV (AcNPV). Isozyme analysis showed that the cells had distinctive patterns from other insect cell lines.
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© 2000 by the Japanese Society of Applied Entomology and Zoology
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