抄録
Mature larvae of the flesh fly, Boettcherisca peregrina, ligated at the tenth abdominal segment in the last larval stage showed a disturbance of puparium formation (pupariation) in the anterior portion. The anterior portion of the larval body was injected with samples tested. The disturbance of pupariation in the anterior portion was dose-dependently rescued by a single injection of ecdysteroids. The minimum effective doses of exogenous ecdysteroids to induce puparial tanning at the anterior portion were 125, 64, 64, 32, 16 and 8 ng for inokosterone, ecdysone, 20-hydroxyecdysone-22acetate, ponasterone A, 20-hydroxyecdysone and polypodine B, respectively. This assay showed a similar ecdysteroid-sensitivity to a conventional bioassay but produced results approximately 2 days earlier than the conventional assay that employed the isolated abdomen of B. peregrina larvae ligated at the sixth body segment.
